About: Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection

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About: Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection
Creator	Lee, Taek Park, Chulhwan Hong, Keonyoung Kim, Younghun Min, Junhong Sohn, Hiesang Kim, Ga Kim, Soo
Source	Elsevier; Medline; PMC
abstract	Abstract In the present study, we fabricated a label-free avian influenza (AIV H5N1) detection biosensor composed of a multi-functional DNA 3 way-Junction (3 W J) on a hollow Au spike-like nanoparticle (hAuSN) using a localized surface plasmon resonance (LSPR) method. To construct the multi-functional DNA (MF-DNA) as a bioprobe, the 3 W J was introduced. The proposed AIV detection bioprobe should contain three functionalities: target recognition, signal amplification, and connection to substrate. To achieve this goal, each piece of the DNA 3 W J was tailored to a hemagglutinin (HA) binding aptamer, FAM dye and thiol group, respectively. The assembly of each DNA 3 W J functional fragment was then confirmed by TBM-Native PAGE. Moreover, the hAuSN was immobilized on the indium-tin-oxide (ITO) substrate for LSPR measurement. The DNA 3 W J was immobilized onto the hAuSN electrode through the thiol-group of DNA 3 W J. The fabricated DNA 3 W J/hAuSN heterolayer on the ITO substrate was investigated by field emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). LSPR experiments were conducted to confirm HA protein binding to the DNA 3 W J/ hAuSN -modified electrode. The proposed biosensor can detected the HA protein in PBS buffer (LOD: 1 pM) as well as in the diluted chicken serum (LOD: 1 pM). The present study details a label-free, simple fabrication method consisted of DNA 3 W J/ hAuSN heterolayer that uses easy-to-tailor elements to detect not only AIV but also various viruses detection platform easily.
has issue date	2019-10-01(xsd:dateTime)
bibo:doi	10.1016/j.colsurfb.2019.06.070
bibo:pmid	31284148
has license	els-covid
sha1sum (hex)	73e97c80da805cb7452f25adb5f946559b5aed18
schema:url	https://doi.org/10.1016/j.colsurfb.2019.06.070
resource representing a document's title	Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection
has PubMed Central identifier	PMC7185628
has PubMed identifier	31284148
schema:publication	Colloids and Surfaces B: Biointerfaces
resource representing a document's body	covid:73e97c80da805cb7452f25adb5f946559b5aed18#body_text
is schema:about of	named entity 'electrode' named entity 'detection' named entity 'VIRUS DETECTION' named entity 'MULTI' named entity 'AVIAN INFLUENZA' named entity 'RECOGNITION' named entity 'SIGNAL AMPLIFICATION' named entity 'TBM' named entity 'EXPERIMENTS' named entity 'NANOPARTICLE' named entity 'H5N1' named entity 'TAILOR' named entity 'FAM' named entity 'BINDING' named entity 'CONFIRMED BY' named entity 'PRESENT' named entity 'SUBSTRATE' named entity 'MODIFIED' named entity 'CHICKEN SERUM' named entity 'PLATFORM' named entity 'APTAMER' named entity 'VARIOUS' named entity 'INTRODUCED' named entity 'COMPOSED OF' named entity 'BIOPROBE' named entity 'FUNCTIONAL' named entity 'BUT' named entity 'FUNCTIONALITIES' named entity 'SEM' named entity 'FRAGMENT' named entity 'MULTI' named entity 'DNA' named entity 'NATIVE PAGE' named entity 'PBS BUFFER' named entity 'PROTEIN BINDING' named entity 'ELEMENTS' named entity 'ELECTRODE' named entity 'HOLLOW' named entity 'AVIAN INFLUENZA VIRUS' named entity 'LABEL-FREE' named entity 'DNA' named entity 'BIOSENSOR' named entity 'TAILORED' named entity 'FIELD EMISSION SCANNING ELECTRON MICROSCOPY' named entity 'OXIDE' named entity '283' named entity 'STUDY' named entity 'INDIUM' named entity 'DETECTED' named entity 'EASY' named entity 'ITO' named entity 'TIN' named entity 'COMPOSED OF' named entity 'NANOPARTICLES' named entity 'FUNCTIONAL' named entity 'LIKE' named entity 'PIECE' named entity 'OF EACH' named entity 'LIKE' named entity 'HOLLOW' named entity 'SPIKE' named entity 'SURFACE PLASMON RESONANCE BIOSENSOR' named entity 'LOCALIZED' named entity 'LABEL-FREE' named entity 'THIOL GROUP' named entity 'DETAILS' named entity 'CONNECTION' named entity 'TARGET' named entity 'CONTAIN' named entity 'DILUTED' named entity 'HEMAGGLUTININ' named entity 'GOAL' named entity 'USING' named entity 'DETECTION'

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