About: Treatment of acute lung inflammation by pulmonary delivery of anti-TNF-α siRNA with PAMAM dendrimers in a murine model

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About: Treatment of acute lung inflammation by pulmonary delivery of anti-TNF-α siRNA with PAMAM dendrimers in a murine model Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Treatment of acute lung inflammation by pulmonary delivery of anti-TNF-α siRNA with PAMAM dendrimers in a murine model
Creator	Yang, Mingshi Foged, Camilla Andreana, Ilaria Bohr, Adam Fattal, Elias Tsapis, Nicolas
Source	Elsevier; Medline; PMC
abstract	Abstract To improve the efficacy of nucleic acid-based therapeutics, e.g., small interfering RNA (siRNA), transfection agents are needed for efficient delivery into cells. Several classes of dendrimers have been found useful as transfection agents for the delivery of siRNA because their surface can readily be functionalized, and the size of the dendriplexes they form with siRNA is within the range of conventional nanomedicine. In this study, commercially available generation 3 poly(amidoamine) (PAMAM) dendrimer was investigated for pulmonary delivery of siRNA directed against tumor necrosis factor (TNF) α for the treatment of acute lung inflammation. Delivery efficiency was assessed in vitro in the RAW264.7 macrophage cell line activated with lipopolysaccharide (LPS), and efficacy was evaluated in vivo in a murine model of LPS-induced lung inflammation upon pre-treatment with TNF-α siRNA. The PAMAM dendrimer-siRNA complexes (dendriplexes) displayed strong siRNA condensation and high cellular uptake in macrophages compared with non-complexed siRNA. Q-PCR analyses showed that the dendriplexes mediated efficient and specific TNF-α silencing in vitro, as compared to non-complexed siRNA and dendriplexes with negative control siRNA. Also in vivo, the PAMAM dendriplexes induced efficacious TNF-α siRNA inhibition, as compared to non-complexed siRNA, upon pulmonary administration to mice with LPS-induced lung inflammation. Hence, these data suggest that PAMAM dendrimers are promising for the local delivery of TNF-α siRNA in the treatment of lung inflammation via pulmonary administration.
has issue date	2020-08-13(xsd:dateTime)
bibo:doi	10.1016/j.ejpb.2020.08.009
bibo:pmid	32798665
has license	els-covid
sha1sum (hex)	7386641bc3dcbc4ebb31513e13b10a072d0e8b3e
schema:url	https://doi.org/10.1016/j.ejpb.2020.08.009
resource representing a document's title	Treatment of acute lung inflammation by pulmonary delivery of anti-TNF-α siRNA with PAMAM dendrimers in a murine model
has PubMed Central identifier	PMC7425770
has PubMed identifier	32798665
schema:publication	European Journal of Pharmaceutics and Biopharmaceutics
resource representing a document's body	covid:7386641bc3dcbc4ebb31513e13b10a072d0e8b3e#body_text
is schema:about of	named entity 'DELIVERY' named entity 'siRNA' named entity 'A. Bohr' named entity 'TNF-α' named entity 'TNF-α' named entity 'lung inflammation' named entity 'streptomycin' named entity 'dendrimers' named entity 'TNF-α' named entity 'siRNA' named entity 'inflammatory response' named entity 'lung inflammation' named entity 'siRNA' named entity 'denaturation' named entity 'phosphate' named entity 'Student's t-test' named entity 'lung inflammation' named entity 'negative control' named entity 'Germany' named entity 'TNF-α' named entity 'Promega' named entity 'TNF-α' named entity 'culture plates' named entity 'siRNA' named entity 'LPS' named entity 'flow cytometry' named entity 'siRNA' named entity 'siRNA' named entity 'inflammation' named entity 'siRNA' named entity 'protein' named entity 'phosphate' named entity 'mice' named entity 'primers' named entity 'ethidium bromide' named entity 'dendrimers' named entity 'numerical aperture' named entity 'fetal bovine serum' named entity 'non-invasive' named entity 'lung inflammation' named entity 'mRNA silencing' named entity 'TNF-α' named entity 'antisense' named entity 'siRNA' named entity 'cationic' named entity 'negative control' named entity 'agarose gels' named entity 'LPS' named entity 'phosphate' named entity 'statistical significance' named entity 'cytokines' named entity 'Thermo Fisher' named entity 'dynamic light scattering' named entity 'catheter' named entity 'therapeutic targets' named entity 'relative humidity' named entity 'siRNA' named entity 'mRNA expression' named entity 'PBS' named entity 'siRNA' named entity 'nmol' named entity 'asthma' named entity 'pro-inflammatory cytokines' named entity 'cellular uptake' named entity 'negative control' named entity 'siRNA' named entity 'TNF-α' named entity 'TRIzol' named entity 'Eurogentec' named entity 'dendrimer' named entity 'zeta potential' named entity 'Real-time PCR' named entity 'animal experiments' named entity 'Laboratory Animal' named entity 'stress levels' named entity 'qPCR'

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