About: Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay

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About: Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
Creator	Talbot, P Johnson, G Adachi, D Ayers, M Draker, R Mazzulli, T Tellier, R
Source	Elsevier; Medline; PMC
abstract	The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada.
has issue date	2004-12-01(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2004.07.008
bibo:pmid	15488617
has license	no-cc
sha1sum (hex)	7210b8b9a235e200c8f787e604334549e3b3584c
schema:url	https://doi.org/10.1016/j.jviromet.2004.07.008
resource representing a document's title	Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
has PubMed Central identifier	PMC7112817
has PubMed identifier	15488617
schema:publication	J Virol Methods
resource representing a document's body	covid:7210b8b9a235e200c8f787e604334549e3b3584c#body_text
is schema:about of	named entity 'sequencing' named entity 'assay' named entity 'IDENTIFICATION' named entity 'ETIOLOGIC AGENT' named entity 'HUMAN CORONAVIRUS' named entity 'DESCRIBED' named entity 'CORONAVIRUSES' named entity 'SENSITIVITY' named entity 'TORONTO' named entity 'OUTBREAK' named entity 'STUDY' named entity 'RT-PCR ASSAY' named entity 'OBTAINED' named entity 'SAMPLES' named entity 'RESPIRATORY' named entity 'TUBE' named entity 'OC43' covid:arg/7210b8b9a235e200c8f787e604334549e3b3584c named entity 'RT-PCR ASSAY' named entity 'HUMAN' named entity 'KNOWN' named entity 'USEFUL' named entity 'DETECTION' named entity 'INCLUDING' named entity 'DETECT' named entity 'PROVIDED' named entity 'NEWLY' named entity 'SEQUENCING' named entity 'HUMAN' named entity 'CORONAVIRUSES' named entity 'SARS-ASSOCIATED CORONAVIRUS' named entity 'COMPREHENSIVE' named entity 'PRESENTS' named entity 'HCOV-229E' named entity 'VIRUS' named entity 'IS A' named entity 'SINGLE' named entity 'INCLUDING' named entity 'ANALYSIS' named entity 'AMPLICON' named entity 'SINGLE' named entity 'SARS' named entity 'ESTABLISHED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'SPECIES IDENTIFICATION' named entity 'RESTRICTION ENZYME ANALYSIS' named entity 'HIGH' named entity 'RAPID SCREENING TEST' named entity 'ANALYTICAL' named entity 'CANADA' named entity 'ASSOCIATED' named entity 'human' named entity 'amplicon' named entity 'HCoV-OC43' named entity 'SARS' named entity 'human coronavirus' named entity 'SARS' named entity 'human coronaviruses' named entity 'amplicon' named entity 'neighbor joining' named entity 'conserved regions' named entity 'enzyme' named entity 'PCR' named entity 'viruses' named entity 'RNA' named entity 'throat swab' named entity 'virus' named entity 'amino acid sequence' named entity 'RNA' named entity 'RT-PCR' named entity 'ORF' named entity 'coronaviruses' named entity 'PCR' named entity 'primers' named entity 'amplicon'

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