About: An immunoprobe to measure Rubisco concentrations and maximal photosynthetic rates of individual phytoplankton cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An immunoprobe to measure Rubisco concentrations and maximal photosynthetic rates of individual phytoplankton cells
Creator	Orellana, Mhica Perry, Mary
Source	PMC
abstract	The cross‐reactivity of an immunological probe to the key photosynthetic enzyme Rubisco (ribulose‐1,5‐bisphosphate carboxylase/oxygenase) was characterized as part of a larger effort to determine maximal photosynthetic rates of individual phytoplankton cells. Polyclonal antiserum was produced against purified Rubisco from the marine diatom Chaetoceros gracilis. The results of western immunoblotting demonstrated that the antiserum reacted positively with Rubisco from 38 species of algae and higher plants and failed to react with only three species of dinoflagellates and one prochlorophyte species. However, the binding affinity or the strength of the cross‐reaction for the polyclonal antiserum with purified Rubisco varied among species. The antiserum was then affinity purified against spinach Rubisco and its binding affinity for purified Rubisco determined by ELISA. Two taxonomic groupings resulted: one with high‐binding affinity (these species included chrysophytes, bacillariophytes, prymnesiophytes, and chlorophytes) and the other with low‐binding affinity (dinophytes and cyanophytes). Rubisco concentration per cell and light‐saturated rates of photosynthesis were highly correlated for cultures of the diatom Thalassiosira weissflogii. These results indicate that affinity‐purified antiserum can be rigorously characterized for use in quantifying Rubisco concentration and for assessing the maximal photosynthetic potential of individual phytoplankton cells.
has issue date	2003-12-22(xsd:dateTime)
bibo:doi	10.4319/lo.1992.37.3.0478
bibo:pmid	32336786
has license	no-cc
sha1sum (hex)	71f0a5a3d6da30b779af91f5edc12011554da8aa
schema:url	https://doi.org/10.4319/lo.1992.37.3.0478
resource representing a document's title	An immunoprobe to measure Rubisco concentrations and maximal photosynthetic rates of individual phytoplankton cells
has PubMed Central identifier	PMC7165624
has PubMed identifier	32336786
schema:publication	Limnol Oceanogr
resource representing a document's body	covid:71f0a5a3d6da30b779af91f5edc12011554da8aa#body_text
is schema:about of	named entity 'failed' named entity 'However' named entity 'spinach' named entity 'purified' named entity 'species' named entity 'phytoplankton' named entity 'concentrations' named entity 'STRENGTH' named entity 'BINDING AFFINITY' named entity 'CROSS' named entity 'SATURATED' named entity 'CELLS' named entity 'RUBISCO' named entity 'CHARACTERIZED' named entity 'USE' named entity 'ELISA' named entity 'SPINACH' named entity 'individual' named entity 'species' named entity 'photosynthesis' named entity 'immunoblotting' named entity 'purified' named entity 'reacted' named entity 'cells' named entity 'produced' named entity 'antiserum' named entity 'Two' named entity 'photosynthetic rates' named entity 'Rubisco' named entity 'photosynthetic' named entity 'dinoflagellates' named entity 'spinach' named entity 'binding affinity' named entity 'algae' named entity 'binding affinity' named entity 'phytoplankton' named entity 'Rubisco' named entity 'bacillariophytes' named entity 'Rubisco' named entity 'quantifying' named entity 'spinach' named entity 'Gonyaulax' named entity 'PBS' named entity 'marine alga' named entity 'Rubisco' named entity 'antiserum' named entity 'BSA' named entity 'fluorescence' named entity 'dinoflagellate' named entity 'coccoid' named entity 'dinoflagellates' named entity 'nutrient' named entity 'phytoplankton' named entity 'Rubisco' named entity 'Rubisco' named entity 'Rubisco' named entity 'Monoclonal antibodies' named entity 'Dinoflagellates' named entity 'Rubisco' named entity 'photosynthesis' named entity 'w/v' named entity 'Statistical information' named entity 'nitrocellulose' named entity 'marine phytoplankton' named entity 'Rubisco' named entity 'antisera' named entity 'Rubisco' named entity 'photons' named entity 'Dunaliella salina' named entity 'paraformaldehyde' named entity 'photosynthetic' named entity 'antiserum' named entity 'spinach' named entity 'chrysophytes' named entity 'antiserum' named entity 'SDS-PAGE' named entity 'antibodies' named entity 'BSA' named entity 'BSA' named entity 'primary production'

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