About: Characterization and immunogenicity of norovirus capsid-derived virus-like particles purified by anion exchange chromatography

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About: Characterization and immunogenicity of norovirus capsid-derived virus-like particles purified by anion exchange chromatography Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization and immunogenicity of norovirus capsid-derived virus-like particles purified by anion exchange chromatography
Creator	Blazevic, • Huhti, L Tamminen, • Vesikari, •
Source	Medline; PMC
abstract	Recombinant baculovirus (BV) expression systems are widely applied in the production of viral capsid proteins and virus-like particles (VLPs) for use as immunogens and vaccine candidates. Traditional density gradient purification of VLPs does not enable complete elimination of BV-derived impurities, including live viruses, envelope glycoprotein gp64 and baculoviral DNA. We used an additional purification system based on ionic strength to purify norovirus (NoV) GII-4 capsid-derived VLPs. The anion exchange chromatography purification led to highly purified VLPs free from BV impurities with intact morphology. In addition, highly purified VLPs induced strong NoV-specific antibody responses in BALB/c mice. Here, we describe a method for NoV VLP purification and several methods for determining their purity, including quantitative PCR for BV DNA detection.
has issue date	2012-12-11(xsd:dateTime)
bibo:doi	10.1007/s00705-012-1565-7
bibo:pmid	23229011
has license	no-cc
sha1sum (hex)	7199ccf73298f72b106f9b3b07abe28b8b56f80d
schema:url	https://doi.org/10.1007/s00705-012-1565-7
resource representing a document's title	Characterization and immunogenicity of norovirus capsid-derived virus-like particles purified by anion exchange chromatography
has PubMed Central identifier	PMC7087179
has PubMed identifier	23229011
schema:publication	Arch Virol
resource representing a document's body	covid:7199ccf73298f72b106f9b3b07abe28b8b56f80d#body_text
is schema:about of	named entity 'highly' named entity 'purified' named entity 'norovirus' named entity 'production' named entity 'DERIVED' named entity 'CHARACTERIZATION' named entity 'ADDITIONAL' named entity 'IMMUNOGENS' named entity 'IMMUNOGENICITY' named entity 'INDUCED' named entity 'FREE' named entity 'METHODS' named entity 'PURIFICATION' named entity 'ANION EXCHANGE CHROMATOGRAPHY' named entity 'NOV' named entity 'VIRUSES' named entity 'PRODUCTION' named entity 'PURITY' named entity 'CAPSID' named entity 'CAPSID PROTEINS' named entity 'VACCINE' named entity 'IONIC STRENGTH' named entity 'PURIFIED' named entity 'ENABLE' named entity 'DOES NOT' named entity 'INTACT' named entity 'METHOD' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'LIVE' named entity 'SYSTEM' named entity 'DNA' named entity 'BACULOVIRUS' named entity 'ADDITION' named entity 'VLPS' named entity 'VIRUS-LIKE PARTICLES' named entity 'CAPSID' named entity 'PURIFIED' named entity 'THEIR' named entity 'SYSTEMS' named entity 'STRONG' named entity 'NOROVIRUS' named entity 'LED' named entity 'ANION EXCHANGE CHROMATOGRAPHY' named entity 'APPLIED' named entity 'QUANTITATIVE PCR' named entity 'GII' named entity 'USE' named entity 'BASED' named entity 'VIRAL CAPSID' named entity 'NOROVIRUS' named entity 'SPECIFIC ANTIBODY' named entity 'RESPONSES' named entity 'HIGHLY' named entity 'DERIVED' named entity 'HERE' named entity 'INCLUDING' named entity 'MICE' named entity 'DESCRIBE' named entity 'MORPHOLOGY' named entity 'USED' named entity 'VLP' named entity 'VIRUS-LIKE PARTICLES' named entity 'DENSITY GRADIENT' named entity 'DETECTION' named entity 'EXPRESSION' named entity 'RECOMBINANT' named entity 'COMPLETE' named entity 'BALB' named entity 'ELIMINATION' named entity 'IMPURITIES' named entity 'TRADITIONAL' covid:arg/7199ccf73298f72b106f9b3b07abe28b8b56f80d named entity 'non-infectious' named entity 'intradermally' named entity 'Sf9' named entity 'specific immune response' named entity 'VLP' named entity 'Protein'

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