About: Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection
Creator	Xia, Ningshao Wang, Jin Zhang, Li, Tingdong Chen, Zhongfu Su, Xiaosong Wang, Yingbin Yang, Kunyu Xu, Jiasu Zhang, Shiyin Zhang, Dongxu Zhong, Zecheng Ge A#, Shengxiang
Source	BioRxiv
abstract	A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] in Wuhan, China, which then rapidly spread globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks. Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity. Additive-supplemented PCR mixes were freeze-dried. The residual moisture of the freeze-dried PCR mixes was measured by Karl-Fischer titration. We found that freeze-dried PCR mixes with ∼1.2% residual moisture are optimal for storage, transport, and reconstitution. The sensitivity, specificity, and repeatability of the freeze-dried reagents were similar to those of freshly prepared, wet reagents. The freeze-dried mixes retained activity at room temperature (18∼25°C) for 28 days, and for 14 and 10 days when stored at 37°C and 56°C, respectively. The uptake of this approach will ease logistical challenges faced by transport networks and make more cold storage space available at diagnosis and hospital laboratories. This method can also be applied to the generation of freeze-dried PCR mixes for the detection of other pathogens.
has issue date	2020-04-09(xsd:dateTime)
bibo:doi	10.1101/2020.04.07.029934
has license	biorxiv
sha1sum (hex)	716ab77ee8c48b8f72d559bd882334a4b50e24cf
schema:url	https://doi.org/10.1101/2020.04.07.029934
resource representing a document's title	Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection
schema:publication	bioRxiv
resource representing a document's body	covid:716ab77ee8c48b8f72d559bd882334a4b50e24cf#body_text
is schema:about of	named entity 'enable' named entity 'logistics' named entity 'ambient temperatures' named entity 'coronavirus disease 2019' named entity 'titration' named entity 'PCR' named entity 'pneumonia' named entity 'transport' named entity 'SARS-CoV-2' named entity 'specificity' named entity 'PCR' named entity 'outbreak' named entity 'COVID' named entity 'optimal' named entity 'diagnosis' named entity 'sensitivity' named entity 'mixes' named entity 'residual' named entity 'reaction' named entity 'PCR' named entity 'freeze-dried' named entity 'hospital laboratories' named entity 'PCR' named entity 'Wuhan' named entity 'freeze-dried' named entity 'PCR-based' named entity 'SARS-CoV-2' named entity 'PCR' named entity 'Room-temperature' named entity 'Molecular Diagnosis' named entity 'PCR' named entity 'drying' named entity 'freeze-dried' named entity 'PCR' named entity 'freeze-dried' named entity 'room temperature' named entity 'freeze-dried' named entity 'reagent' named entity 'PCR' named entity 'single-stranded' named entity 'ambient temperature' named entity '28 days' named entity 'RNA virus' named entity 'PCR' named entity 'lyophilized' named entity 'assay' named entity 'rRT-PCR' named entity 'reverse transcriptase' named entity 'PCR' named entity 'lyophilization' named entity 'mycobacteria' named entity 'serial dilution' named entity 'lyophilization' named entity 'freeze-dried' named entity 'storage effect' named entity 'nucleic acid' named entity 'PCR' named entity 'freeze-dried' named entity 'lyophilized' named entity 'pathogens' named entity 'molecular technique' named entity 'reagent' named entity 'freeze-dried' named entity 'ice crystal' named entity 'PCR' named entity 'freeze-dried' named entity 'freeze-dried' named entity 'freeze-dried' named entity 'lyophilized' named entity 'room temperature' named entity '28 days' named entity 'lyophilization'

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