About: Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR

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About: Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR
Creator	Buonavoglia, Canio Decaro, Nicola Desario, Costantina Elia, Gabriella Martella, Vito Lorusso, Eleonora Cavalli, Alessandra Lucente, Maria
Source	Elsevier; Medline; PMC
abstract	A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was used for generation of standard RNAs. The detection limit of the assay was 1 × 10(2) RNA copies and standard curve displayed a linear range from 1 × 10(2) to 1 × 10(9) copies and a good reproducibility. The assay was then applied to determine the mRNA loads in the tissues of dogs naturally infected by CPV-2. mRNA was detected in a variety of tissues, including the central nervous system.
has issue date	2007-08-10(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2007.06.017
bibo:pmid	17692932
has license	no-cc
sha1sum (hex)	71355d22c1570abb0e9d250b892e540b2c0a68f3
schema:url	https://doi.org/10.1016/j.jviromet.2007.06.017
resource representing a document's title	Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR
has PubMed Central identifier	PMC7112852
has PubMed identifier	17692932
schema:publication	J Virol Methods
resource representing a document's body	covid:71355d22c1570abb0e9d250b892e540b2c0a68f3#body_text
is schema:about of	named entity 'naturally' named entity 'targeting' named entity 'parvovirus' named entity 'type' named entity 'real-time' named entity 'INFECTIOUS' named entity 'REPRODUCIBILITY' named entity 'DETERMINE' named entity 'LINEAR RANGE' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'ASSEMBLY' named entity 'REAL-TIME RT-PCR' named entity 'PROBE' named entity 'CPV' named entity 'RT-PCR ASSAY' named entity 'DNA FRAGMENT' named entity 'REAL-TIME RT-PCR' named entity 'TAQMAN' named entity 'GENERATION' named entity 'DETECTED' covid:arg/71355d22c1570abb0e9d250b892e540b2c0a68f3 named entity 'TYPE 2' named entity 'DETECTION' named entity 'REPLICATING' named entity 'ASSAY' named entity 'CANINE PARVOVIRUS' named entity 'MRNA' named entity 'TRANSCRIPTS' named entity 'USED' named entity 'SPLICED' named entity 'MIMIC' named entity 'DETECTION LIMIT' named entity 'PAIR' named entity 'COPIES' named entity 'BASED' named entity 'TARGETING' named entity 'RNA' named entity 'GOOD' named entity 'GENE' named entity 'MRNA' named entity 'DETECTION' named entity 'DOGS' named entity 'DISPLAYED' named entity 'SYNTHETIC DNA' named entity 'PCR' named entity 'NS2 MRNA' named entity 'TISSUES' named entity 'PRIMERS' named entity 'APPLIED' named entity 'CONSTRUCTED' named entity 'CURVE' named entity 'VARIETY' named entity 'STANDARD' named entity 'INCLUDING' named entity 'INFECTED' named entity 'pair' named entity 'range' named entity 'assay' named entity 'loads' named entity 'tissues' named entity 'central nervous' named entity 'mRNA' named entity 'TaqMan' named entity 'reproducibility' named entity 'primers' named entity 'sensitive and specific' named entity 'virus' named entity 'PCR' named entity '2005a' named entity 'neurons' named entity 'canine' named entity 'amino acid' named entity 'oligonucleotide' named entity 'real-time RT-PCR'

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