About: Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles

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About: Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles
Creator	Liu, Min Pan, Wei Zhao, Ping Qi, Zhong-Tian Zhao, Lan-Juan Lu, Yang Cao, Jie Ke, Jin-Shan Qin, Zhao-Ling
Source	Elsevier; Medline; PMC
abstract	SARS-CoV is a newly identified coronavirus that causes severe acute respiratory syndrome (SARS). Currently, there is no effective method available for prophylaxis and treatment of SARS-CoV infections. In the present study, the influence of small interfering RNA (siRNA) on SARS-CoV nucleocapsid (N) protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS-CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein (EGFP) fusion protein were observed. A candidate siRNA was proved to down-regulate N and EGFP expression actively in a sequence-specific manner. The expression vector of this siRNA was constructed and confirmed to reduce N and EGFP expression efficiently in both cultured cells and adult mouse muscles. Our findings suggest that the siRNA should provide the basis for prophylaxis and therapy of SARS-CoV infection in human.
has issue date	2005-04-25(xsd:dateTime)
bibo:doi	10.1016/j.febslet.2005.02.080
bibo:pmid	15848179
has license	no-cc
sha1sum (hex)	7032a9d4be6168b5ea9d7c50126fcc09a79e25fc
schema:url	https://doi.org/10.1016/j.febslet.2005.02.080
resource representing a document's title	Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles
has PubMed Central identifier	PMC7094369
has PubMed identifier	15848179
schema:publication	FEBS Lett
resource representing a document's body	covid:7032a9d4be6168b5ea9d7c50126fcc09a79e25fc#body_text
is schema:about of	named entity 'cultured cells' named entity 'siRNA' named entity 'green' named entity 'reduce' named entity 'NUCLEOCAPSID' named entity 'HUMAN' named entity 'MOUSE' named entity 'THEIR' named entity 'CANDIDATE' named entity 'ENHANCED' named entity 'PROTEIN EXPRESSION' named entity 'EXPRESSION VECTOR' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'DRIVEN' named entity 'PROTEIN ' named entity 'BASIS' named entity 'CONFIRMED' named entity 'GENE EXPRESSION' named entity 'MUSCLES' named entity 'INFLUENCE' named entity 'PRESENT' named entity 'SARS-COV' named entity 'MUSCLES' named entity 'MOUSE' named entity 'NUCLEOCAPSID' named entity 'CULTURED CELLS' named entity 'SARS-COV' named entity 'SMALL INTERFERING RNA' named entity 'EFFECTIVE' named entity 'SPECIFIC' named entity 'PROMOTER' named entity 'DETECTED' named entity 'FINDINGS' named entity 'SEQUENCE' named entity 'N GENE' named entity 'PROPHYLAXIS' named entity 'INHIBITORY' named entity 'THERAPY' named entity 'GREEN ' named entity 'OBSERVED' named entity 'INFECTIONS' named entity 'SIRNA' named entity 'TREATMENT' named entity 'SUGGEST' named entity 'NEWLY' named entity 'TARGETING' named entity 'CORONAVIRUS' named entity 'METHOD' named entity 'PROVIDE' named entity 'CAUSES' named entity 'STUDY' named entity 'AVAILABLE' named entity 'EGFP' named entity 'TO REDUCE' named entity 'CULTURED CELLS' named entity 'CASSETTES' named entity 'SMALL INTERFERING RNA' named entity 'REGULATE' named entity 'OUR' named entity 'CURRENTLY' named entity 'PREPARED' named entity 'EFFECTS' named entity 'IS A' named entity 'CONSTRUCTED' named entity 'IDENTIFIED' named entity 'EXPRESSION' named entity 'ADULT MOUSE' named entity 'FUSION PROTEIN' named entity 'FLUORESCENCE PROTEIN' named entity 'mouse' named entity 'prophylaxis' named entity 'siRNA' named entity 'SARS-CoV'

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