About: Isolation and characterization of WUPyV in polarized human airway epithelial cells

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About: Isolation and characterization of WUPyV in polarized human airway epithelial cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and characterization of WUPyV in polarized human airway epithelial cells
Creator	Wang, Chao Song, Jingdong Guo, Qiong Xie, Zhiping Zheng, Lishu Chen, Aijun Huang, Yiman Wei, Tianli
Source	Medline; PMC
abstract	BACKGROUND: Washington University polyomavirus (WUPyV) is a novel human polyomavirus detected in childwith acute respiratory infection in 2007. However, the relationship between WUPyV and respiratory diseases has yet to be established for lacking of a suitable in vitro culture system. METHODS: To isolate WUPyV with human airway epithelial (HAE) cells, the positive samples were incubated in HAE, and then the nucleic acid, VP1 protein and virions were detected using real-time PCR, immunofluorescence and electron microscopy respectively. RESULTS: The result showed that WUPyV could replicate effectively in HAE cells and virions with typical polyomavirus characteristics could be observed. Additionally, the entire genome sequence of the isolated strain (BJ0771) was obtained and phylogenetic analysis indicated that BJ0771 belongs to gene cluster I. CONCLUSIONS: Our findings demonstrated clinical WUPyV strain was successfully isolated for the first time in the world and this will help unravel the etiology and pathogenic mechanisms of WUPyV in respiratory infection diseases.
has issue date	2020-07-09(xsd:dateTime)
bibo:doi	10.1186/s12879-020-05224-y
bibo:pmid	32646445
has license	cc-by
sha1sum (hex)	6fbfb1e1af70fa96f8e9a699c114ad5c34dfe23b
schema:url	https://doi.org/10.1186/s12879-020-05224-y
resource representing a document's title	Isolation and characterization of WUPyV in polarized human airway epithelial cells
has PubMed Central identifier	PMC7344044
has PubMed identifier	32646445
schema:publication	BMC Infect Dis
resource representing a document's body	covid:6fbfb1e1af70fa96f8e9a699c114ad5c34dfe23b#body_text
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