About: Putative Receptor Binding Domain of Bat-Derived Coronavirus HKU9 Spike Protein: Evolution of Betacoronavirus Receptor Binding Motifs

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About: Putative Receptor Binding Domain of Bat-Derived Coronavirus HKU9 Spike Protein: Evolution of Betacoronavirus Receptor Binding Motifs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Putative Receptor Binding Domain of Bat-Derived Coronavirus HKU9 Spike Protein: Evolution of Betacoronavirus Receptor Binding Motifs
Creator	Wu, Ying Lu, Guangwen Wang, Qihui Qi, Jianxun Yan, Jinghua Huang, Canping Yuan, Yuan Gao, George Zhang, Yanfang
Source	Medline; PMC
abstract	[Image: see text] The suggested bat origin for Middle East respiratory syndrome coronavirus (MERS-CoV) has revitalized the studies of other bat-derived coronaviruses with respect to interspecies transmission potential. Bat coronavirus (BatCoV) HKU9 is an important betacoronavirus (betaCoV) that is phylogenetically affiliated with the same genus as MERS-CoV. The bat surveillance data indicated that BatCoV HKU9 has been widely spreading and circulating in bats. This highlights the necessity of characterizing the virus for its potential to cross species barriers. The receptor binding domain (RBD) of the coronavirus spike (S) protein recognizes host receptors to mediate virus entry and is therefore a key factor determining the viral tropism and transmission capacity. In this study, the putative S RBD of BatCoV HKU9 (HKU9-RBD), which is homologous to other betaCoV RBDs that have been structurally and functionally defined, was characterized via a series of biophysical and crystallographic methods. By using surface plasmon resonance, we demonstrated that HKU9-RBD binds to neither SARS-CoV receptor ACE2 nor MERS-CoV receptor CD26. We further determined the atomic structure of HKU9-RBD, which as expected is composed of a core and an external subdomain. The core subdomain fold resembles those of other betaCoV RBDs, whereas the external subdomain is structurally unique with a single helix, explaining the inability of HKU9-RBD to react with either ACE2 or CD26. Via comparison of the available RBD structures, we further proposed a homologous intersubdomain binding mode in betaCoV RBDs that anchors the external subdomain to the core subdomain. The revealed RBD features would shed light on the evolution route of betaCoV.
has issue date	2016-10-03(xsd:dateTime)
bibo:doi	10.1021/acs.biochem.6b00790
bibo:pmid	27696819
has license	bronze-oa
sha1sum (hex)	6f7d0062b880f92aacdf5f51f48278d4782f1817
schema:url	https://doi.org/10.1021/acs.biochem.6b00790
resource representing a document's title	Putative Receptor Binding Domain of Bat-Derived Coronavirus HKU9 Spike Protein: Evolution of Betacoronavirus Receptor Binding Motifs
has PubMed Central identifier	PMC7075523
has PubMed identifier	27696819
schema:publication	Biochemistry
resource representing a document's body	covid:6f7d0062b880f92aacdf5f51f48278d4782f1817#body_text
is schema:about of	named entity 'EVOLUTION' named entity 'RBD' named entity 'RBDs' named entity 'positive-stranded' named entity 'affiliated' named entity 'subdomain' named entity 'genus' named entity 'DOMAIN' named entity 'STUDY' named entity 'COMPARISON' named entity 'FOLD' named entity 'REVEALED' named entity 'BINDS' named entity 'INDICATED' named entity 'STUDIES' named entity 'ATOMIC STRUCTURE' named entity 'SPECIES' named entity 'GENUS' named entity 'STRUCTURES' named entity 'SINGLE' named entity 'TRANSMISSION' named entity '28S' named entity 'CD26' named entity 'LIGHT' named entity 'NECESSITY' named entity 'RECEPTOR BINDING' named entity 'BAT' covid:arg/6f7d0062b880f92aacdf5f51f48278d4782f1817 named entity 'DOMAIN' named entity 'UNIQUE' named entity 'MODE' named entity 'FEATURES' named entity 'DEMONSTRATED' named entity 'MOTIFS' named entity 'SPIKE PROTEIN' named entity 'BETACORONAVIRUS' named entity 'DERIVED' named entity 'CORONAVIRUS' named entity 'DERIVED' named entity 'AVAILABLE' named entity 'RECEPTORS' named entity 'HAVE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'EITHER' named entity 'ROUTE' named entity 'SPREADING' named entity 'BAT' named entity 'VIRUS ENTRY' named entity 'BIOPHYSICAL' named entity 'ACE2' named entity 'ITS' named entity 'HOST' named entity 'BINDING' named entity 'DATA' named entity 'USING' named entity 'SUBDOMAIN' named entity 'POTENTIAL' named entity 'VIRUS' named entity 'CORONAVIRUSES' named entity 'CORONAVIRUS' named entity 'SURFACE PLASMON RESONANCE' named entity 'PROTEIN ' named entity 'INABILITY' named entity 'NEITHER' named entity 'RBD' named entity 'RECEPTOR BINDING' named entity 'DETERMINED' named entity 'IMPORTANT' named entity 'EVOLUTION' named entity 'RECEPTOR' named entity 'SURVEILLANCE' named entity 'REACT' named entity 'METHODS'

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