About: Interferon-Induced Transmembrane Protein 3 Blocks Fusion of Diverse Enveloped Viruses by Locally Altering Mechanical Properties of Cell Membranes

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About: Interferon-Induced Transmembrane Protein 3 Blocks Fusion of Diverse Enveloped Viruses by Locally Altering Mechanical Properties of Cell Membranes Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Interferon-Induced Transmembrane Protein 3 Blocks Fusion of Diverse Enveloped Viruses by Locally Altering Mechanical Properties of Cell Membranes
Creator	Li, Xiang Marin, Mariana Lu, Wuyuan Guo, Xiangyang Melikyan, Gregory Steinkühler, Jan
Source	BioRxiv
abstract	Interferon-induced transmembrane protein 3 (IFITM3) potently inhibits entry of diverse enveloped viruses by trapping the viral fusion at a hemifusion stage, but the underlying mechanism remains unclear. Here, we show that recombinant IFITM3 reconstituted into lipid vesicles induces negative membrane curvature and that this effect maps to its small amphipathic helix (AH). We demonstrate that AH: (i) partitions into lipid-disordered domains where IAV fusion occurs, (ii) induces negative membrane curvature, and (iii) increases lipid order and membrane stiffness. Additionally, replacing the IFITM3 AH with AH from an unrelated protein did not compromise its antiviral activity. These effects on membrane properties correlate with the fusion-inhibitory activity, as exogenous addition of AH to insulin-producing cells reduces glucose-stimulated insulin secretion. Our results thus imply that IFITM3 inhibits the transition from hemifusion to full fusion by imposing an unfavorable membrane curvature and increasing the order and stiffness of the cytoplasmic leaflet of endosomal membranes.
has issue date	2020-06-26(xsd:dateTime)
bibo:doi	10.1101/2020.06.25.171280
has license	biorxiv
sha1sum (hex)	6f76d1a1308cf70d4f59e4666ea6b85d82fd889b
schema:url	https://doi.org/10.1101/2020.06.25.171280
resource representing a document's title	Interferon-Induced Transmembrane Protein 3 Blocks Fusion of Diverse Enveloped Viruses by Locally Altering Mechanical Properties of Cell Membranes
schema:publication	bioRxiv
resource representing a document's body	covid:6f76d1a1308cf70d4f59e4666ea6b85d82fd889b#body_text
is schema:about of	named entity 'membrane curvature' named entity 'lipid' named entity 'inhibits' named entity 'Membranes' named entity 'stiffness' named entity 'recombinant' named entity 'fusion' named entity 'amphipathic' named entity 'IFITM3' named entity 'lipid' named entity 'vesicles' named entity 'Lyso' named entity 'Lo phase' named entity 'lipid' named entity 'polar solvent' named entity 'biological membrane' named entity 'phase separation' named entity 'dye' named entity 'IFITM3' named entity 'IFITM3' named entity 'protein' named entity 'cholesterol' named entity 'IFITM1' named entity 'dye' named entity 'IFITM3' named entity 'cleavage' named entity 'ganglioside' named entity 'S1C' named entity 'room temperature' named entity 'Lingwood' named entity 'lipid' named entity 'vesicles' named entity 'emission spectrum' named entity 'IFITM3' named entity 'oil-immersion objective' named entity 'vacuum chamber' named entity 'solubilization' named entity 'synthetic membranes' named entity 'solvent' named entity 'IAV' named entity 'IFITM3' named entity 'Laurdan' named entity 'phosphatidylethanolamine' named entity 'GM1' named entity 'endosomal' named entity 'IFITM3' named entity 'S4C' named entity 'assay' named entity 'cytoplasmic region' named entity 'fluorescent' named entity 'liposomes' named entity 'Virus' named entity 'IAV' named entity 'generalized polarization' named entity 'peptide' named entity 'cellular membranes' named entity 'helical' named entity 'HEPES' named entity 'peptide' named entity 'phase-separated' named entity 'POPC' named entity 'IFITM3' named entity 'IFITM3' named entity 'lipid oxidation' named entity 'IAV' named entity 'IFITM3' named entity 'substrate' named entity 'single-phase' named entity 'phase boundary' named entity 'room temperature' named entity 'cytoplasmic' named entity 'cholesterol' named entity 'peptides' named entity 'Laurdan' named entity 'sine wave' named entity 'viruses' named entity 'protein' named entity 'membrane curvature' named entity 'GM1'

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