About: Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs

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About: Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs
Creator	Chen, Qi Yoon, Kyoung-Jin Gauger, Phillip Harmon, Karen Sinha, Avanti Zhang, Jianqiang Giménez-Lirola, Luis Zimmerman, Jeffrey Meng, Xiang-Jin Madson, Darin Burrough, Eric Magstadt, Drew Thomas, Joseph Welch, Michael Salzbrenner, Holly
Source	Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV) was identified in the United States (U.S.) swine population for the first time in April 2013 and rapidly spread nationwide. However, no information has been published regarding the minimum infectious dose (MID) of PEDV in different pig models. The main objective of this study was to determine the oral minimum infectious dose of PEDV in naïve conventional neonatal piglets and weaned pigs. A U.S. virulent PEDV prototype isolate (USA/IN19338/2013) with known infectious titer was serially ten-fold diluted in virus-negative cell culture medium. Dilutions with theoretical infectious titers from 560 to 0.0056 TCID(50)/ml together with a medium control were orogastrically inoculated (10ml/pig) into 7 groups of 5-day-old neonatal pigs (n = 4 per group) and 7 groups of 21-day-old weaned pigs (n = 6 per group). In 5-day-old pigs, 10ml of inoculum having titers 560–0.056 TCID(50)/ml, corresponding to polymerase chain reaction (PCR) cycle threshold (Ct) values 24.2–37.6, resulted in 100% infection in each group; 10ml of inoculum with titer 0.0056 TCID(50)/ml (Ct>45) caused infection in 25% of the inoculated pigs. In 21-day-old pigs, 10ml of inoculum with titers 560–5.6 TCID(50)/ml (Ct 24.2–31.4) resulted in 100% infection in each group while 10ml of inoculum with titers 0.56–0.0056 TCID(50)/ml (Ct values 35.3 –>45) did not establish infection in any pigs under study conditions as determined by clinical signs, PCR, histopathology, immunohistochemistry, and antibody response. These data reveal that PEDV infectious dose is age-dependent with a significantly lower MID for neonatal pigs compared to weaned pigs. This information should be taken into consideration when interpreting clinical relevance of PEDV PCR results and when designing a PEDV bioassay model. The observation of such a low MID in neonates also emphasizes the importance of strict biosecurity and thorough cleaning/disinfection on sow farms.
has issue date	2015-10-06(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0139266
bibo:pmid	26441071
has license	cc-by
sha1sum (hex)	6f06d1fd7c4ae1397b0a8f5192e9f0c7dc5e62df
schema:url	https://doi.org/10.1371/journal.pone.0139266
resource representing a document's title	Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs
has PubMed Central identifier	PMC4594914
has PubMed identifier	26441071
schema:publication	PLoS One
resource representing a document's body	covid:6f06d1fd7c4ae1397b0a8f5192e9f0c7dc5e62df#body_text
is schema:about of	named entity 'OUTCOMES' named entity 'CONVENTIONAL' covid:arg/6f06d1fd7c4ae1397b0a8f5192e9f0c7dc5e62df named entity 'NEONATAL' named entity 'TITER' named entity 'NEONATAL' named entity 'CLEANING' named entity 'DILUTED' named entity 'CONVENTIONAL' named entity '5.6' named entity 'ORAL' named entity 'DIFFERENT' named entity 'MINIMUM' named entity 'VALUES' named entity 'GROUPS' named entity 'REVEAL' named entity 'THESE' named entity 'FARMS' named entity 'IDENTIFIED' named entity 'INFECTIOUS DOSE' named entity 'AGE' named entity 'APRIL' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'OBSERVATION' named entity 'PUBLISHED' named entity 'ISOLATE' named entity 'VIRUS' named entity 'CELL CULTURE' named entity 'CLINICAL SIGNS' named entity 'UNITED STATES' named entity 'DETERMINED BY' named entity 'BIOASSAY' named entity 'THEORETICAL' named entity 'DEPENDENT' named entity 'PCR' named entity 'LOWER' named entity 'MID' named entity 'HISTOPATHOLOGY' named entity '100%' named entity 'CULTURE MEDIUM' named entity 'STUDY' named entity 'FOLD' named entity 'INOCULUM' named entity 'INFECTION' named entity 'PIGS' named entity 'INTERPRETING' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'DAY-OLD' named entity 'NEONATES' named entity 'VIRULENT' named entity 'RAPIDLY' named entity 'WEANED' named entity 'INFORMATION' named entity 'DATA' named entity 'INFECTION' named entity 'PIGS' named entity 'ANTIBODY RESPONSE' named entity 'CLINICAL RELEVANCE' named entity 'WEANED' named entity 'INFECTIOUS' named entity 'POLYMERASE CHAIN REACTION' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'DETERMINE' named entity 'CYCLE THRESHOLD' named entity 'INFECTIOUS' named entity 'CONDITIONS' named entity 'CONSIDERATION'

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