About: Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

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About: Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
Creator	Tong, Suxiang Lowe, Luis Erdman, Dean Emery, Shannon Bankamp, Bettina Anderson, Larry Bellini, William Bowen, Michael Cook, Byron Holloway, Brian Ksiazek, Tom Meyer, Richard Newton, Bruce Rota, Paul Winchell, Jonas Mccaustland, Karen
source	Medline; PMC
abstract	A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
has issue date	2004-02-01(xsd:dateTime)
bibo:doi	10.3201/eid1002.030759
bibo:pmid	15030703
has license	no-cc
sha1sum (hex)	6e6b122886cf7d009604cdd36ef743ddb632a9c1
schema:url	https://doi.org/10.3201/eid1002.030759
resource representing a document's title	Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
has PubMed Central identifier	PMC3322901
has PubMed identifier	15030703
schema:publication	Emerg Infect Dis
resource representing a document's body	covid:6e6b122886cf7d009604cdd36ef743ddb632a9c1#body_text
is schema:about of	named entity 'GenBank' named entity 'GENOME' named entity 'RT-PCR' named entity 'ASSOCIATED' named entity 'PRIMER' named entity 'COPIES' named entity 'CORONAVIRUS' named entity 'POTENTIAL' named entity 'DETECT' named entity 'ISOLATION' named entity 'DETECTION LIMIT' named entity 'SPECIMENS' named entity 'CLINICAL' named entity 'ASSAY' named entity 'MULTIPLE' named entity 'FORWARD PRIMER' named entity 'LOCATION' named entity 'REGIONS' named entity 'POLYMERASE CHAIN REACTION' named entity 'AID' named entity 'GENOMIC' named entity 'CULTURE' named entity 'STRAIN' named entity 'REVERSE TRANSCRIPTION' named entity 'SUITABLE' named entity 'BASED' named entity 'APPLICATION' named entity 'CORONAVIRUSES' named entity 'DIAGNOSING' named entity 'RT-PCR ASSAY' named entity 'SENSITIVE' named entity 'REAL-TIME' named entity 'ANIMAL' named entity 'LOCATED IN' named entity 'DIFFERENT' named entity 'HUMAN' named entity 'PROBE' named entity 'REVERSE PRIMER' named entity 'DISCRIMINATE' named entity 'ACCESSION' named entity 'REACTION' named entity '3C10' named entity 'RAPIDLY' named entity 'TO DETECT' named entity 'SARS-COV' named entity 'REAL-TIME RT-PCR' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'PCR' named entity 'CONVENTIONAL' covid:arg/6e6b122886cf7d009604cdd36ef743ddb632a9c1 named entity 'SARS-CoV' named entity 'sets' named entity 'acute' named entity 'coronavirus' named entity 'infection' named entity 'severe acute respiratory syndrome' named entity 'severe acute respiratory syndrome' named entity 'primer' named entity 'RT-PCR' named entity 'based' named entity 'RT-PCR' named entity 'sensitive, and specific' named entity 'real-time RT-PCR' named entity 'SARS' named entity 'etiologic agent' named entity 'Primer'

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