About: An RNA Aptamer That Specifically Binds to the Glycosylated Hemagglutinin of Avian Influenza Virus and Suppresses Viral Infection in Cells

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About: An RNA Aptamer That Specifically Binds to the Glycosylated Hemagglutinin of Avian Influenza Virus and Suppresses Viral Infection in Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An RNA Aptamer That Specifically Binds to the Glycosylated Hemagglutinin of Avian Influenza Virus and Suppresses Viral Infection in Cells
Creator	Kim, Dong-Eun Kwon, Hyun-Mi Han, Mi Kim, Dong Lee, Woo, Byung Kwang, Hyun
Source	PMC
abstract	The influenza virus surface glycoprotein hemagglutinin (HA) is responsible for viral attachment to sialic acid-containing host cell receptors and it facilitates the initial stage of viral infection. In the present study, we isolated an RNA aptamer specific to the glycosylated receptor-binding domain of the HA protein (gHA1) after 12 cycles of the systematic evolution of ligands by exponential enrichment procedure (SELEX), and we then investigated if the selected aptamer suppresses viral infection in host cells. Nitrocellulose filter binding and enzyme-linked immunosorbent assay (ELISA) experiments revealed that 1 RNA aptamer, HA12-16, bound specifically to the gHA1 protein. Cell viability assay showed that the HA12-16 RNA aptamer suppressed viral infection in host cells by enhancing cell viability. Immunofluorescence microscopic analysis further demonstrated that the HA12-16 RNA aptamer suppresses viral attachment to host cells by neutralizing the receptor-binding site of influenza virus HA. These results indicate that the isolated RNA aptamer can be developed as an antiviral reagent against influenza through appropriate therapeutic formulation.
has issue date	2014-05-16(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0097574
bibo:pmid	24835440
has license	cc-by
sha1sum (hex)	6e4a162ab67f96a6b889e1f09d5531770fb1996d
schema:url	https://doi.org/10.1371/journal.pone.0097574
resource representing a document's title	An RNA Aptamer That Specifically Binds to the Glycosylated Hemagglutinin of Avian Influenza Virus and Suppresses Viral Infection in Cells
has PubMed Central identifier	PMC4023947
has PubMed identifier	24835440
schema:publication	PLoS One
resource representing a document's body	covid:6e4a162ab67f96a6b889e1f09d5531770fb1996d#body_text
is schema:about of	named entity 'domain' named entity 'viability assay' named entity 'influenza' named entity 'glycoprotein' named entity 'virus' named entity 'Infection' named entity 'Viral' named entity 'HEMAGGLUTININ' named entity 'INVESTIGATED' named entity 'HOST CELLS' named entity 'REVEALED' named entity 'ANTIVIRAL' named entity 'INITIAL STAGE' named entity 'FORMULATION' named entity 'THERAPEUTIC' named entity 'PROCEDURE' named entity 'PRESENT' named entity 'BOUND' named entity 'SELECTED' named entity 'THESE' named entity 'REAGENT' named entity 'SUPPRESSES' named entity 'BINDING SITE' named entity 'GLYCOSYLATED' named entity 'INFLUENZA VIRUS' named entity 'ANALYSIS' named entity 'APPROPRIATE' named entity 'SPECIFIC' named entity 'CELL VIABILITY ASSAY' named entity 'SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT' named entity 'RECEPTOR' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'VIRAL INFECTION' named entity 'MICROSCOPIC' named entity 'HA PROTEIN' named entity 'VIRAL ATTACHMENT' named entity 'INFLUENZA' named entity 'VIRAL INFECTION' named entity 'EXPERIMENTS' named entity 'BINDS' named entity 'PROTEIN' named entity 'NITROCELLULOSE' named entity 'GHA1' named entity 'HEMAGGLUTININ' named entity 'RNA APTAMER' named entity 'SUPPRESSES' named entity 'CELLS' named entity 'AVIAN INFLUENZA VIRUS' named entity 'SITE OF' named entity 'IMMUNOFLUORESCENCE' named entity 'RESULTS' named entity 'HOST CELL' named entity 'RNA APTAMER' named entity 'FILTER BINDING' named entity 'STUDY' named entity 'RESPONSIBLE' named entity 'ENHANCING' named entity 'PROTEIN ' named entity 'DOMAIN' named entity 'CONTAINING' named entity 'DEMONSTRATED' named entity 'CELL VIABILITY' named entity 'GLYCOSYLATED' named entity 'ISOLATED' named entity 'APTAMER' named entity 'RECEPTORS' named entity 'BINDING' named entity 'SIALIC ACID' named entity 'SUPPRESSED' covid:arg/6e4a162ab67f96a6b889e1f09d5531770fb1996d named entity 'host' named entity 'glycosylated' named entity 'RNA' named entity 'hemagglutinin' named entity 'facilitates'

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