About: Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions

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About: Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions
Creator	Zhang, Yan Li, Ping He, Chao Shen, Wen-Long Shi, Ming-Lei Xie, De-Jian Zhang, Xiang-Yuan Zhang, Zhang Zhao, Zhi-Hu
Source	Elsevier; Medline; PMC
abstract	Abstract Interferon-induced transmembrane protein (IFITM) 1, 2 and 3 genes encode a family of interferon (IFN)-induced transmembrane proteins that block entry of a broad spectrum of pathogens. However, the transcriptional regulation of these genes, especially whether there exist any enhancers and their roles during the IFN induction process remain elusive. Here, through public data mining, episomal luciferase reporter assay and in vivo CRISPR-Cas9 genome editing, we identified an IFN-responsive enhancer located 35kb upstream of IFITM3 gene promoter upregulating the IFN-induced expression of IFITM1, 2 and 3 genes. Chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA) and luciferase reporter assay demonstrated that signal transducers and activators of transcription (STAT) 1 bound to the enhancer with the treatment of IFN and was indispensable for the enhancer activity. Furthermore, using chromosome conformation capture technique, we revealed that the IFITM1, 2 and 3 genes physically clustered together and constitutively looped to the distal enhancer through long-range interactions in both HEK293 and A549 cells, providing structural basis for coordinated regulation of IFITM1, 2 and 3 by the enhancer. Finally, we showed that in vivo truncation of the enhancer impaired IFN-induced resistance to influenza A virus (IAV) infection. These findings expand our understanding of the mechanisms underlying the transcriptional regulation of IFITM1, 2 and 3 expression and its ability to mediate IFN signaling.
has issue date	2017-08-31(xsd:dateTime)
bibo:doi	10.1016/j.bbagrm.2017.05.003
bibo:pmid	28511927
has license	els-covid
sha1sum (hex)	6d68f39c881d1617b562bcf67cfa42cc442615c1
schema:url	https://doi.org/10.1016/j.bbagrm.2017.05.003
resource representing a document's title	Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions
has PubMed Central identifier	PMC7102783
has PubMed identifier	28511927
schema:publication	Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
resource representing a document's body	covid:6d68f39c881d1617b562bcf67cfa42cc442615c1#body_text
is schema:about of	named entity 'basis' named entity 'induction' named entity 'transcriptional regulation' named entity 'EMSA' named entity 'ChIP' named entity 'IFN' named entity 'IFN' named entity 'CHROMATIN' named entity 'INDUCTION' named entity 'PUBLIC' named entity 'ENHANCER' named entity 'ACTIVITY' named entity 'COORDINATED' named entity 'CHROMATIN IMMUNOPRECIPITATION' named entity 'DISTAL' named entity 'TRANSDUCERS' named entity 'IDENTIFIED' named entity 'UNDERLYING' named entity 'REVEALED' named entity 'TECHNIQUE' named entity 'LONG' named entity 'REPORTER ASSAY' named entity 'IFN' named entity 'INDISPENSABLE' named entity 'GENES' named entity 'RESISTANCE TO' named entity 'ENHANCERS' named entity 'INTERFERON-INDUCED TRANSMEMBRANE PROTEIN' named entity 'ENTRY' named entity 'CRISPR-CAS9' named entity 'IFITM1' named entity 'STRUCTURAL' named entity 'INDUCED RESISTANCE' named entity 'IMPAIRED' named entity 'INFECTION' named entity 'INTERACTIONS' named entity 'RANGE' named entity 'RESPONSIVE' named entity 'EXIST' named entity 'GENE PROMOTER' named entity 'FINDINGS' named entity 'HERE' named entity 'TRUNCATION' named entity 'IFITM1' named entity 'REGULATION' named entity 'LONG' named entity 'GENES' named entity 'COORDINATED' named entity 'ITS' named entity 'EXPRESSION' named entity 'INFLUENZA A VIRUS' named entity 'THEIR' named entity 'ROLES' named entity 'GENOME EDITING' named entity 'MECHANISMS' named entity 'UNDERSTANDING' named entity 'INTERACTIONS' named entity 'CHROMOSOME CONFORMATION CAPTURE' named entity 'SIGNAL' named entity 'TRANSMEMBRANE PROTEINS' named entity 'MEDIATE' named entity 'EXPAND' named entity 'THESE' named entity 'BASIS' named entity 'TREATMENT' named entity 'ENCODE' named entity 'REGULATION' named entity 'LUCIFERASE' named entity 'SIGNALING' named entity 'ENHANCER' named entity 'ELECTROPHORETIC MOBILITY SHIFT ASSAY ' named entity 'DATA MINING' named entity 'TRANSCRIPTIONAL REGULATION'

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