About: Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control

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About: Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control
Creator	Zhang, Yi Ma, Xuejun Fan, Tao Wang, Ruihuan Li, Lixin Li, Xinna Pan, Jing Qi, Juju Shen, Xinxin Song, Guowei Song, · Cn, Maxj@ivdc Fan, · Shen, ·
Source	Medline; PMC
abstract	Human respiratory syncytial virus (RSV) is a common viral pathogen that causes lower respiratory tract infections in infants and children globally. In this study, we developed a duplex reverse transcription recombinase-aided amplification (duplex-rtRAA) assay containing an internal control in a single closed tube for the detection of human RSV. The internal control in the amplification effectively eliminated false-negative results and ensured the accuracy of the duplex-rtRAA system. We first developed and evaluated a universal singleplex-rtRAA assay for RSV. The sensitivity of this assay for RSV was determined as 4.4 copies per reaction, and the specificity was 100%. Next, a duplex-rtRAA assay with an internal control was established. The sensitivity of the duplex-rtRAA assay approached 5.0 copies per reaction, and no cross-reaction with other common respiratory viruses was observed. The two detection methods (singleplex-rtRAA and duplex-rtRAA) developed in this study were used to test 278 clinical specimens, and the results showed absolute consistency with RSV RT-qPCR analysis, demonstrating 100% diagnostic sensitivity and specificity. These data indicate that the duplex-rtRAA has great potential for the rapid detection of RSV with a high sensitivity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-019-04230-z) contains supplementary material, which is available to authorized users.
has issue date	2019-05-03(xsd:dateTime)
bibo:doi	10.1007/s00705-019-04230-z
bibo:pmid	31053978
has license	no-cc
sha1sum (hex)	6ca3f120df064f4cf573e1d8d60ee81cd1ed68e7
schema:url	https://doi.org/10.1007/s00705-019-04230-z
resource representing a document's title	Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control
has PubMed Central identifier	PMC7086889
has PubMed identifier	31053978
schema:publication	Arch Virol
resource representing a document's body	covid:6ca3f120df064f4cf573e1d8d60ee81cd1ed68e7#body_text
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