About: The herbal extract deriving from aerial parts of Scutellaria baicalensis shows anti-inflammation and anti-hypoxia responses in cultured fin cells from rabbit fish

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The herbal extract deriving from aerial parts of Scutellaria baicalensis shows anti-inflammation and anti-hypoxia responses in cultured fin cells from rabbit fish Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The herbal extract deriving from aerial parts of Scutellaria baicalensis shows anti-inflammation and anti-hypoxia responses in cultured fin cells from rabbit fish
Creator	Li, Xiao Duan, Ran Hu, Wei-Hui Qin, Qi-Wei Wang, Wen-Xiong Wu, Qi-Yun Xia, Yi-Teng Dong, Ran Dong, Ting-Xia Duan, Jian Li, Qi-Wei Li, Shao-Ping Qin, Wen-Xiong Ting-Xia, Tina Tsim, Tsim, Wah-Keung Wah-Keung, Wah-Keung, Karl Wang, Karl Wang, Qin Q-W Xia, Y-T Xiao, Shao-Ping
Source	Elsevier; Medline; PMC
abstract	A new cell line derived from dorsal fin of rabbit fish Siganus fuscescens was developed and characterized. The cell line was isolated from the dorsal fin, named as rabbit fish fin (RFF) cell line, and which was sub-cultured for 50 cycles since the development. This cell line was tested for growth in different temperatures and serum concentrations, and the best growing condition was at 20% serum at 28 °C. In cultured RFF cells, amplification of 18S rRNA from genomic DNA and immunostaining of cellular cytokeratin confirmed the proper identity of S. fuscescens fish. After 30th passage of cultures, the cells were exposed to challenge of inflammation, triggered by LPS, and hypoxia, mimicked by CoCl(2). Cultured RFF cells showed robust sensitive responses to inflammation and hypoxia in directing the expressions of cytokines and hypoxia inducible factor-1α (HIF-1α). The water extract of aerial part of Scutellaria baicalensis (SBA) has been shown in rabbit fish to prevent inflammation. Here, we extended this notion of testing the efficacy of SBA extract in the developed cultured RFF cells. Application of SBA extract inhibited the expression of LPS-induced inflammatory cytokines, i.e. IL-1β, IL-6, as well as the signaling of NF-κB. The application of CoCl(2) in cultured RFF cells triggered the hypoxia-induced cell death and up regulation of HIF-1α. As expected, applied SBA extract in the cultures prevented the hypoxia-induced signaling. Our results show the established RFF cell line may be served as an ideal in vitro model in drug screening relating to inflammation and hypoxia. Additionally, we are supporting the usage of SBA herbal extract in fish aquaculture, which possesses efficacy against inflammation and hypoxia.
has issue date	2020-07-29(xsd:dateTime)
bibo:doi	10.1016/j.fsi.2020.07.050
bibo:pmid	32738512
has license	no-cc
sha1sum (hex)	68562448e47707c7bd1ba53bf0de325692a0d8dd
schema:url	https://doi.org/10.1016/j.fsi.2020.07.050
resource representing a document's title	The herbal extract deriving from aerial parts of Scutellaria baicalensis shows anti-inflammation and anti-hypoxia responses in cultured fin cells from rabbit fish
has PubMed Central identifier	PMC7387939
has PubMed identifier	32738512
schema:publication	Fish Shellfish Immunol
resource representing a document's body	covid:68562448e47707c7bd1ba53bf0de325692a0d8dd#body_text
is schema:about of	named entity 'TO PREVENT' named entity 'RFF' named entity 'IDENTITY' named entity 'EXPRESSIONS' named entity 'IDEAL' named entity 'EXTRACT' named entity 'AQUACULTURE' named entity 'ISOLATED' named entity 'IMMUNOSTAINING' named entity '20%' named entity 'TEMPERATURES' covid:arg/68562448e47707c7bd1ba53bf0de325692a0d8dd named entity 'Scutellaria baicalensis' named entity 'NF-κB' named entity 'dorsal fin' named entity 'fish fin' named entity 'hypoxia' named entity 'RFF' named entity 'hypoxia' named entity 'herbal extract' named entity 'cell line' named entity 'hypoxia' named entity 'serum' named entity 'RFF' named entity 'hypoxia' named entity 'cytokeratin' named entity 'RFF' named entity 'hypoxia inducible factor' named entity 'IL-1β' named entity 'RFF' named entity 'rabbit fish' named entity 'herbal extract' named entity 'rabbit fish' named entity 'inflammatory response' named entity 'LPS' named entity 'translocation' named entity 'inflammation' named entity 'p65' named entity 'LPS' named entity 'NF-κB' named entity 'Norcross' named entity 'RFF' named entity 'cell survival' named entity 'hypoxia' named entity 'LPS' named entity 'LPS' named entity 'RFF' named entity 'NF-kB' named entity 'RFF' named entity 'translocation' named entity 'cell death' named entity 'RFF' named entity 'transfected' named entity '20%' named entity 'dose-dependent manner' named entity 'IL-6' named entity 'LPS' named entity 'p65' named entity 'RFF' named entity 'transfected'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software