About: Mechanism of Methylprednisolone‐Induced Primary Cilia Formation Disorder and Autophagy in Osteoblasts

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About: Mechanism of Methylprednisolone‐Induced Primary Cilia Formation Disorder and Autophagy in Osteoblasts Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mechanism of Methylprednisolone‐Induced Primary Cilia Formation Disorder and Autophagy in Osteoblasts
Creator	Bai, Rui Guo, Shi-Bing Liu, Wan-Lin Yan, Jing-Long Zhao, Zhen-Qun
Source	Medline; PMC
abstract	OBJECTIVE: To study the role of primary cilia formation disorder and osteoblasts autophagy in the pathogenesis of steroid‐induced avascular necrosis of the femoral head (SANFH). METHODS: Osteoblasts were isolated from rabbit bones and treated with 1 μM Methylprednisolone for 0, 12, 24, 48, and 72 h. The Beclin1, MAP1LC3, Atg‐5, Atg‐12, IFT20 and OFD1 mRNAs and proteins were detected by PCR and Western blotting, and their correlation was statistically analyzed. The lengths of osteoblast cilia were measured under a laser confocal microscope, and the autophagy flux was tracked by transfecting the osteoblasts with GFP‐RFP‐LC3 lentivirus. RESULTS: Methylprednisolone significantly upregulated Beclin1, MAP1LC3, Atg‐5, Atg‐12 and OFD1 mRNAs and proteins in a time‐dependent manner, and decreased that of IFT20 (P < 0.05). In addition, the autophagy flux in the osteoblasts also increased and the ciliary length decreased in a time‐dependent manner after Methylprednisolone treatment. The length of the cilia were 5.46 ± 0.11 um at 0 h, 4.08 ± 0.09 um at 12 h, 3.07 ± 0.07 um at 24 h, 2.31 ± 0.10 um at 48 h, and finally 1.15 ± 0.04 um at 72 h. Methylprednisolone treatment also affects primary cilium numbers in cultures, for 0 to 72 h. The autophagy regulatory genes, Beclin1, MAP1LC3, Atg‐5 and Atg‐12, were found to be negatively correlated with IFT20, with an average correlation coefficient of −0.81. A negative correlation was also found between OFD1 and IFT20, with an average correlation coefficient of −0.53. CONCLUSION: Methylprednisolone inhibits primary cilia formation and promotes autophagy, which could be the pathological basis of SANFH. The exact regulatory mechanism needs to be further studied in vivo.
has issue date	2020-02-16(xsd:dateTime)
bibo:doi	10.1111/os.12630
bibo:pmid	32064763
has license	cc-by-nc
sha1sum (hex)	681a4dcd3ab332a84758adae27e1266e62e974aa
schema:url	https://doi.org/10.1111/os.12630
resource representing a document's title	Mechanism of Methylprednisolone‐Induced Primary Cilia Formation Disorder and Autophagy in Osteoblasts
has PubMed Central identifier	PMC7189053
has PubMed identifier	32064763
schema:publication	Orthop Surg
resource representing a document's body	covid:681a4dcd3ab332a84758adae27e1266e62e974aa#body_text
is schema:about of	covid:arg/681a4dcd3ab332a84758adae27e1266e62e974aa named entity 'autophagy' named entity 'study' named entity 'Primary Cilia' named entity 'Methylprednisolone' named entity 'Beclin1' named entity 'China' named entity 'IFT20' named entity 'RT-PCR' named entity 'China' named entity 'physiological' named entity 'chemiluminescence' named entity 'thrice' named entity 'China' named entity 'ischemia' named entity 'Invitrogen' named entity 'Methylprednisolone' named entity 'mechanical stress' named entity 'Shanghai' named entity 'autophagosome' named entity 'cilia' named entity 'primary cilia' named entity 'TRIzol' named entity 'glucocorticoid' named entity 'cell growth' named entity 'phagosome' named entity 'Methylprednisolone' named entity 'primary antibodies' named entity 'gap junction' named entity 'basal' named entity 'GAPDH' named entity 'primary cilia' named entity 'epoxy resin' named entity 'Armonk' named entity 'Total protein' named entity 'OFD1' named entity 'protein' named entity 'cilia' named entity 'Methylprednisolone' named entity 'protein' named entity 'autophagy' named entity 'Bio-Rad' named entity 'one-way analysis of variance' named entity 'autophagy' named entity 'prednisolone' named entity 'cytoplasm' named entity 'positively correlated' named entity 'Dalian' named entity 'primary cilia' named entity 'SYBR Green' named entity 'Shandong' named entity 'steroid' named entity 'correlation coefficient' named entity 'Shanghai' named entity 'Methylprednisolone' named entity 'correlation coefficient' named entity 'China' named entity 'Yizheng' named entity 'primary cilia' named entity 'osteoblasts' named entity 'autophagy' named entity 'China' named entity 'IFT20' named entity 'China' named entity 'autophagosome' named entity 'primary cilia' named entity 'statistically significant' named entity 'electron microscope' named entity 'Jiangsu' named entity 'disability' named entity 'Osteoblasts' named entity 'fluorescence' named entity 'correlation' named entity 'osteoblasts' named entity 'IBM' named entity 'Dalian' named entity 'staining cells'

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