About: Molecular characterization of Cryptosporidium isolates from beef calves under one month of age over three successive years in one herd in western France

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About: Molecular characterization of Cryptosporidium isolates from beef calves under one month of age over three successive years in one herd in western France Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular characterization of Cryptosporidium isolates from beef calves under one month of age over three successive years in one herd in western France
Creator	Chartier, Christophe Paraud, Carine Pors, Isabelle Rieux, Anaïs
Source	Elsevier; Medline; PMC
abstract	Abstract Cohorts of pre-weaned calves were studied for Cryptosporidium infection over three successive years (2010–2012) in one beef cattle herd in western France. Each year 25–34 calves were sampled weekly from 3 days to one month of age in order to characterize oocyst output, Cryptosporidium species and clinical features associated with infection. Faecal samples were screened for the presence of oocysts using immunofluorescence analysis. DNA was extracted from positive samples and a PCR SSU rRNA followed by RFLP or sequencing was performed. For the subtyping of C. parvum, a gp60 PCR was carried out. Regardless of the year, 92–100% of the animals excreted oocysts on at least one sampling date. Depending on the year of observation, the age of highest prevalence varied. In contrast, the peak of excretion was systematically observed almost at the same age (2nd–3rd week of life) with excretion levels ranging from between 100 and 1.7×107 oocysts/g of faeces. Differences concerning clinical signs depending on the year of sampling were observed. Different species patterns were observed, with a predominance of C. bovis in the 1st year and a predominance of C. parvum in the last year. Moreover, two zoonotic subtypes of C. parvum, IIaA15G2R1 and IIaA18G2R1, were recorded in different years. This study shows that, in a given farm, the Cryptosporidium species and C. parvum subtypes identified as well as the prevalence of infection and level of excretion may vary greatly and show distinct patterns according to the year.
has issue date	2014-05-28(xsd:dateTime)
bibo:doi	10.1016/j.vetpar.2014.03.004
bibo:pmid	24685023
has license	els-covid
sha1sum (hex)	6801a1db7db0ead9748768e3ae28f544562bca70
schema:url	https://doi.org/10.1016/j.vetpar.2014.03.004
resource representing a document's title	Molecular characterization of Cryptosporidium isolates from beef calves under one month of age over three successive years in one herd in western France
has PubMed Central identifier	PMC7116914
has PubMed identifier	24685023
schema:publication	Veterinary Parasitology
resource representing a document's body	covid:6801a1db7db0ead9748768e3ae28f544562bca70#body_text
is schema:about of	named entity 'positive' named entity 'gp60' covid:arg/6801a1db7db0ead9748768e3ae28f544562bca70 named entity 'vary' named entity 'Cohorts' named entity 'sampling' named entity 'weekly' named entity 'observed' named entity 'PCR' named entity 'age' named entity 'Faecal' named entity 'C. parvum' named entity 'observed' named entity 'samples' named entity 'beef cattle' named entity 'age' named entity '2011' named entity 'C. parvum' named entity 'species' named entity 'For' named entity 'level' named entity 'C. parvum' named entity 'species' named entity 'life' named entity 'herd' named entity 'RFLP' named entity 'zoonotic' named entity 'France' named entity 'clinical features' named entity 'infection' named entity 'Faecal samples' named entity 'C. parvum' named entity 'subtyping' named entity 'excretion' named entity 'Cryptosporidium' named entity 'excreted' named entity 'oocyst' named entity 'oocysts' named entity 'C. bovis' named entity 'Cryptosporidium' named entity 'excretion' named entity 'Rotavirus' named entity 'PCR-RFLP' named entity 'E. coli' named entity 'excreted' named entity 'Bovine' named entity 'C. bovis' named entity 'zoonotic' named entity 'intensive livestock production' named entity 'C. bovis' named entity 'C. bovis' named entity 'France' named entity 'beef calves' named entity 'infection' named entity 'France' named entity 'sediment' named entity 'neonatal' named entity 'beef calves' named entity 'oocysts' named entity 'dairy calves' named entity 'neonates' named entity 'China' named entity 'diarrhoea' named entity 'nested PCR' named entity 'excretion' named entity 'Coronavirus' named entity 'gene' named entity 'PCR-RFLP' named entity 'infection' named entity 'zoonotic' named entity 'excretion' named entity 'C. andersoni' named entity 'agarose' named entity 'infection' named entity 'disinfectants' named entity 'growth retardation' named entity 'nested PCR' named entity 'clinical signs'

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