About: Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells

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About: Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
Creator	Zhang, Yong Bi, Cong-Ming Cao, Jun-Wei He, Xiao-Ning Li, Zhi-Yan Lv, Ning Peng, Shu-Ying Zhang, Shi-Qiang
Source	Elsevier; Medline; PMC
abstract	Abstract The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was constructed and expressed in the mouse mammary cells (EMT6). The recombinant S protein from culture supernatant of transgenic EMT6 was harvested and immunized BALB/c mice. The results demonstrated recombinant S protein was expressed at high levels in mammary cells by Western blotting and enzyme-linked immunosorbent assay (ELISA) detection. The antibody titer in BALB/c mice following immunization with recombinant S protein was detectable after the first immunization. Maximum titers of antibody (8.86±0.19ng/ml of serum) were attained after the second immunization. In conclusion, the recombinant S protein expressed in mammary cells was able to elicit substantial immunological response against TGEV. This lays the basis for using mammary gland bioreactor generating edible vaccine.
has issue date	2007-09-30(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2007.04.004
bibo:pmid	17532081
has license	els-covid
sha1sum (hex)	670bf5f321381ae161143b5e13d811e099218e82
schema:url	https://doi.org/10.1016/j.virusres.2007.04.004
resource representing a document's title	Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells
has PubMed Central identifier	PMC7114266
has PubMed identifier	17532081
schema:publication	Virus Research
resource representing a document's body	covid:670bf5f321381ae161143b5e13d811e099218e82#body_text
is schema:about of	named entity 'antibody titer' named entity 'mouse' named entity 'recombinant' named entity 'immunization' named entity 'coronavirus' named entity 'EXPRESSION VECTOR' named entity 'CONCLUSION' named entity 'LEVELS' named entity 'DETECTION' named entity 'VIRUS' named entity 'TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS' named entity '28S' named entity 'TRANSGENIC' named entity 'TGEV' named entity 'FOLLOWING' named entity 'ANTIBODY TITER' named entity 'PRESENT' named entity 'EDIBLE VACCINE' named entity 'MAMMARY GLAND' named entity 'RECOMBINANT' named entity 'BIOREACTOR' named entity 'HIGH' named entity 'TO INVESTIGATE' named entity 'CDNA' named entity 'BASIS' named entity 'SECOND' named entity 'LAYS' named entity 'PROTEIN ' named entity 'EXPRESSED' named entity 'ANTIBODY' named entity 'MOUSE' named entity 'SPIKE PROTEIN' named entity 'INDUCED' named entity 'CELLS' named entity 'STUDY' named entity 'MICE' named entity 'SPECIFIC' named entity 'GASTROENTERITIS' named entity '0.19' named entity 'IMMUNIZATION' named entity 'EXPRESSED' named entity 'CONSTRUCTED' named entity 'SERUM' named entity 'ELICIT' named entity 'MAMMARY' named entity 'INDUCED' named entity 'MOUSE' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'MAXIMUM' named entity 'DEMONSTRATED' named entity 'GENE' named entity 'IMMUNIZED' named entity 'CELLS' named entity 'USING' named entity 'CULTURE SUPERNATANT' named entity 'CONTAINING' named entity 'SPIKE' named entity 'S PROTEIN' named entity 'PROTEIN ' named entity 'MAMMARY' named entity 'EMT6' named entity 'RESULTS' named entity 'GENERATING' named entity 'WESTERN BLOTTING' named entity 'DETECTABLE' named entity 'LENGTH' named entity 'IMMUNE RESPONSE' named entity 'IMMUNE RESPONSE' named entity '288' named entity 'BALB' covid:arg/670bf5f321381ae161143b5e13d811e099218e82 named entity 'transgenic' named entity 'protein' named entity 'protein'

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