About: A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus

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About: A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus
Creator	Chen, Chen Zhao, Li Ma, Xue-Jun Wang, Ji Wang, Le Feng, Zhi-Shan Li, Gui-Xia Zhao, Meng-Chuan Duan, Su-Xia Qiu, Fang-Zhou
source	Elsevier; Medline; PMC
abstract	Abstract Respiratory syncytial virus (RSV) causes serious respiratory tract infection worldwide. The relatively low RSV load makes it difficult to detect in frail, elderly, and severely immune-compromised patients. In the present study, we developed a locked nucleic acid–-based 1-tube nested real-time RT-PCR (OTNRT-PCR) assay with the advantages of extremely high sensitivity, facile operability, and less likelihood of cross-contamination. The sensitivity, specificity, and clinical performance of the OTNRT-PCR assay were compared in parallel with a conventional TaqMan probe-based real-time PCR (qRT-PCR) assay and a traditional 2-step nested RT-PCR assay. The limit of detection of the OTNRT-PCR assay was 1.02 × 10−1 TCID50/mL, equivalent to the traditional 2-step nested RT-PCR assay and 25-fold lower than the qRT-PCR assay. Of 616 nasopharyngeal aspirates tested, 143 RSV-negative samples by qRT-PCR were confirmed as positive by sequencing the OTNRT-PCR products. We therefore conclude that OTNRT-PCR is more sensitive than qRT-PCR for detection of RSV in clinical samples.
has issue date	2019-02-28(xsd:dateTime)
bibo:doi	10.1016/j.diagmicrobio.2018.09.001
bibo:pmid	30266400
has license	els-covid
sha1sum (hex)	65e5aa4f16b1920009732ff2456fcba5c8f333f2
schema:url	https://doi.org/10.1016/j.diagmicrobio.2018.09.001
resource representing a document's title	A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus
has PubMed Central identifier	PMC7126397
has PubMed identifier	30266400
schema:publication	Diagnostic Microbiology and Infectious Disease
resource representing a document's body	covid:65e5aa4f16b1920009732ff2456fcba5c8f333f2#body_text
is schema:about of	named entity 'positive' named entity 'locked nucleic acid' named entity 'nasopharyngeal' named entity 'samples' named entity 'respiratory syncytial virus' named entity 'RT-PCR ASSAY' named entity 'LNA' named entity 'TCID50' named entity 'BASED' named entity 'CONFIRMED' named entity 'HIGH SENSITIVITY' named entity 'NEGATIVE' named entity 'LIMIT OF DETECTION' named entity 'CONVENTIONAL' named entity '616' named entity 'REAL-TIME PCR ' named entity 'LOCKED NUCLEIC ACID' named entity 'TUBE' named entity 'CONTAMINATION' named entity '921' named entity 'PATIENTS' named entity 'SENSITIVE' named entity 'RSV' named entity 'SEQUENCING' named entity 'EXTREMELY' named entity 'SAMPLES' named entity 'SPECIFICITY' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'PRODUCTS' named entity 'FOLD' named entity 'RESPIRATORY TRACT INFECTION' named entity 'SENSITIVITY' named entity 'FRAIL' named entity 'QRT-PCR' named entity 'COMPARED' named entity 'TO DETECT' named entity 'LIKELIHOOD' named entity 'NASOPHARYNGEAL' named entity 'SEVERELY' named entity 'PERFORMANCE' named entity 'NESTED' named entity 'DETECTION' named entity 'CONCLUDE' named entity 'CLINICAL' named entity 'PCR' named entity 'LOWER' named entity 'DETECTION' named entity 'RT-PCR ASSAY' named entity 'CROSS' named entity 'ASSAY' named entity 'STUDY' named entity 'MODIFIED' named entity 'TUBE' named entity 'USING' named entity 'REAL-TIME RT-PCR' named entity 'PRIMERS' named entity '143' named entity 'OPERABILITY' named entity 'TESTED' named entity 'IMMUNE' named entity 'PCR ASSAY' named entity 'CAUSES' named entity 'ASPIRATES' named entity 'TAQMAN' named entity 'NESTED' named entity 'POSITIVE' named entity 'ELDERLY' named entity 'PARALLEL' named entity 'STEP' named entity 'REAL-TIME RT-PCR' named entity 'PRESENT' named entity 'TRADITIONAL'

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