About: A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses
Creator	Bai, Jianfa Das, Amaresh Shi, Jishu Liu, Xuming Peddireddi, Lalitha Anderson, Gary Jia, Wei Liu, Yanhua Ma, Guiping Shi, Xiju Sun, Qing Wang, Qin Xu, Lu
Source	Elsevier; Medline; PMC
abstract	Mixed infection with different pathogens is common in swine production systems especially under intensive production conditions. Quick and accurate detection and differentiation of different pathogens are necessary for epidemiological surveillance, disease management and import and export controls. In this study, we developed and validated a panel of multiplex real-time PCR/RT-PCR assays composed of four subpanels, each detects three common swine pathogens. The panel detects 12 viruses or viral serotypes, namely, VSV-IN, VSV-NJ, SVDV, CSFV, ASFV, FMDV, PCV2, PPV, PRV, PRRSV-NA, PRRSV-EU and SIV. Correlation coefficients (R(2)) and PCR amplification efficiencies of all singular and triplex real-time PCR reactions are within the acceptable range. Comparison between singular and triplex real-time PCR assays of each subpanel indicates that there is no significant interference on assay sensitivities caused by multiplexing. Specificity tests on 226 target clinical samples or 4 viral strains and 91 non-target clinical samples revealed that the real-time PCR panel is 100% specific, and there is no cross amplification observed. The limit of detection of each triplex real-time PCR is less than 10 copies per reaction for DNA, and less than 16 copies per reaction for RNA viruses. The newly developed multiplex real-time PCR panel also detected different combinations of co-infections as confirmed by other means of detections.
has issue date	2016-08-06(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2016.08.005
bibo:pmid	27506582
has license	no-cc
sha1sum (hex)	633ee9755d994f4e6f4515a40b507dd81cb17811
schema:url	https://doi.org/10.1016/j.jviromet.2016.08.005
resource representing a document's title	A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses
has PubMed Central identifier	PMC7119729
has PubMed identifier	27506582
schema:publication	J Virol Methods
resource representing a document's body	covid:633ee9755d994f4e6f4515a40b507dd81cb17811#body_text
is schema:about of	named entity 'specific' named entity 'assays' named entity 'Comparison' named entity 'real-time PCR' named entity 'copies' named entity 'multiplex' named entity 'disease management' named entity 'pathogens' named entity 'reactions' named entity 'MEANS' named entity 'PCV2' named entity 'IMPORT AND EXPORT' named entity 'REAL-TIME PCR ' named entity 'RNA VIRUSES' named entity 'FMDV' named entity 'DNA' named entity 'AMPLIFICATION' named entity 'ACCURATE' named entity 'ACCEPTABLE' named entity 'QUICK' named entity 'CLINICAL' named entity 'REACTIONS' named entity 'INTERFERENCE' named entity 'STUDY' covid:arg/633ee9755d994f4e6f4515a40b507dd81cb17811 named entity 'ASFV' named entity 'TARGET' named entity 'EFFICIENCIES' named entity 'DETECTED' named entity 'PANEL' named entity 'PATHOGENS' named entity 'COMBINATIONS' named entity 'COMPARISON' named entity 'COMMON' named entity 'NEWLY' named entity 'MULTIPLEXING' named entity 'PCR' named entity 'CONFIRMED BY' named entity 'OF EACH' named entity '226' named entity 'CORRELATION' named entity 'NECESSARY' named entity 'LESS THAN' named entity 'LIMIT OF DETECTION' named entity 'PRV' named entity 'PRODUCTION' named entity 'NON-TARGET' named entity 'MULTIPLEX REAL-TIME PCR' named entity 'REAL-TIME PCR ' named entity 'COMMON' named entity 'ASSAY' named entity 'SEROTYPES' named entity 'TESTS' named entity 'DETECTION' named entity 'COMPOSED OF' named entity 'REACTION' named entity '100%' named entity 'VIRUSES' named entity 'ASSAY' named entity 'DIFFERENTIATION' named entity 'RANGE' named entity 'REVEALED' named entity 'VSV' named entity 'SAMPLES' named entity 'SYSTEMS' named entity 'CONDITIONS' named entity 'SURVEILLANCE' named entity 'MIXED INFECTION' named entity 'PANEL'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software