About: Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System

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About: Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System
Creator	Liu, Juan Wu, Li Zhao, Yu Liu, Yuxuan Li, Wenjie Chen, Jixuan Gu, Guojing Hu, Xiaoyan Huang, Qingzhou Jiao, Hanwei Li, Bowen Luo, Yichen Shuai, Xuehong Wang, Xinglong Wang, Yidan Zheng, Zonglin Zhou, Zhixiong
Source	PMC
abstract	Brucella ovis infection results in genital damage and epididymitis in rams, placental inflammation and rare abortion in ewes, and neonatal mortality in lambs. However, the mechanism underlying B. ovis infection remains unclear. In the present study, we used prokaryotic transcriptome sequencing to identify the differentially expressed genes (DEGs) between wild-type B. ovis and intracellular B. ovis in RAW264.7 macrophages. Gene ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed, and quantitative reverse transcriptase PCR (qRT-PCR) was used to validate the top 10 upregulated and downregulated DEGs. The results showed that 212 genes were differentially expressed, including 68 upregulated and 144 downregulated genes, which were mainly enriched in 30 GO terms linked to biological process, cellular component, and molecular function. KEGG analysis showed that the DEGs were enriched in the hypoxia-inducible factor 1 (HIF-1) signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, beta-alanine metabolism, and quorum sensing pathway. BME_RS01160, BME_RS04270, BME_RS08185, BME_RS12880, BME_RS25875, predicted_RNA865, and predicted_RNA953 were confirmed with the transcriptome sequencing data. Hence, our findings not only reveal the intracellular parasitism of B. ovis in the macrophage immune system, but also help to understand the mechanism of chronic B. ovis infection.
has issue date	2020-05-19(xsd:dateTime)
bibo:doi	10.1007/s10753-020-01239-4
bibo:pmid	32430895
has license	no-cc
sha1sum (hex)	6338469316411cc22656054a138ac914837f96df
schema:url	https://doi.org/10.1007/s10753-020-01239-4
resource representing a document's title	Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System
has PubMed Central identifier	PMC7235551
has PubMed identifier	32430895
schema:publication	Inflammation
resource representing a document's body	covid:6338469316411cc22656054a138ac914837f96df#body_text
is schema:about of	named entity 'The' named entity 'inflammation' named entity 'genes' named entity 'ENRICHED' named entity 'CONFIRMED' named entity 'QUANTITATIVE REVERSE TRANSCRIPTASE PCR' named entity '144' named entity 'REMAINS' named entity 'MECHANISM' named entity 'EXPRESSED' named entity 'MACROPHAGE' named entity 'PCR' named entity 'BETA-ALANINE METABOLISM' covid:arg/6338469316411cc22656054a138ac914837f96df named entity 'quorum' named entity 'Genes' named entity 'remains' named entity 'identify' named entity 'ewes' named entity 'upregulated' named entity 'HIF-1' named entity 'placental' named entity 'neonatal mortality' named entity 'However' named entity 'Macrophage' named entity 'B. ovis' named entity 'B. ovis' named entity 'Gene ontology' named entity 'KEGG' named entity 'mitogen-activated protein kinase' named entity 'cellular component' named entity 'B. ovis' named entity 'quorum sensing' named entity 'intracellular parasitism' named entity 'downregulated' named entity 'quantitative reverse transcriptase PCR' named entity 'qRT-PCR' named entity 'Gene ontology' named entity 'Gene ontology' named entity 'KEGG' named entity 'Intracellular' named entity 'protein' named entity 'internal control' named entity 'reference genome' named entity 'molecular entity' named entity 'B. ovis' named entity '5'UTR' named entity 'abscissa' named entity 'Brucella' named entity 'B. ovis' named entity 'intracellular' named entity 'operon' named entity 'intracellular parasitism' named entity 'B. ovis' named entity 'Macrophages' named entity 'homologous' named entity 'B. ovis' named entity 'protein' named entity 'FDR' named entity 'sequence similarity' named entity 'biological functions' named entity 'secretion' named entity 'WHO' named entity 'GenBank' named entity 'gene expression'

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