About: Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane

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About: Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane
Creator	L Y N N P U D D I N G T O N, Carolyn Rose, K
Source	Medline; PMC
abstract	Oligonucleotide-directed mutagenesis was used to construct chimeric cDNAs that encode the extracellular and transmembrane domains of the vesicular stomatitis virus glycoprotein (G) linked to the cytoplasmic domain of either the immunoglobulin mu membrane heavy chain, the hemagglutinin glycoprotein of influenza virus, or the small glycoprotein (p23) of infectious bronchitis virus. Biochemical analyses and immunofluorescence microscopy demonstrated that these hybrid genes were correctly expressed in eukaryotic cells and that the hybrid proteins were transported to the plasma membrane. The rate of transport to the Golgi complex of G protein with an immunoglobulin mu membrane cytoplasmic domain was approximately sixfold slower than G protein with its normal cytoplasmic domain. However, this rate was virtually identical to the rate of transport of micron heavy chain molecules measured in the B cell line WEHI 231. The rate of transport of G protein with a hemagglutinin cytoplasmic domain was threefold slower than wild type G protein and G protein with a p23 cytoplasmic domain, which were transported at similar rates. The combined results underscore the importance of the amino acid sequence in the cytoplasmic domain for efficient transport of G protein to the cell surface. Also, normal cytoplasmic domains from other transmembrane glycoproteins can substitute for the G protein cytoplasmic domain in transport of G protein to the plasma membrane. The method of constructing precise hybrid proteins described here will be useful in defining functions of specific domains of viral and cellular integral membrane proteins.
has issue date	1986-06-01(xsd:dateTime)
bibo:pmid	3011809
has license	cc-by-nc-sa
sha1sum (hex)	6328beb961554b05276359e9c5b26d2e57cd52e9
resource representing a document's title	Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane
has PubMed Central identifier	PMC2114239
has PubMed identifier	3011809
schema:publication	J Cell Biol
resource representing a document's body	covid:6328beb961554b05276359e9c5b26d2e57cd52e9#body_text
is schema:about of	named entity 'INTEGRAL MEMBRANE PROTEINS' named entity 'CYTOPLASMIC' named entity 'TRANSPORT' named entity 'PLASMA MEMBRANE' named entity 'SUBSTITUTE' named entity 'GLYCOPROTEIN' named entity 'VIRAL' named entity 'OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS' named entity 'CYTOPLASMIC DOMAIN' named entity 'VESICULAR STOMATITIS VIRUS' named entity 'CELLULAR' named entity 'mutagenesis' named entity 'Cytoplasmic' named entity 'Viral' named entity 'Oligonucleotide' named entity 'Cytoplasmic Domain' named entity 'Viral' named entity 'Vesicular Stomatitis Virus' named entity 'methionine' named entity 'protein' named entity 'glycoprotein' named entity 'Golgi' named entity 'cytoplasmic domain' named entity 'glycoprotein' named entity 'cytoplasmic domain' named entity 'electrophoresis' named entity 'cytoplasmic' named entity '15,000' named entity 'plasmid' named entity 'cell surface' named entity 'expression vector' named entity 'IgM' named entity 'protein' named entity 'bacteriophage' named entity 'glycoprotein' named entity 'protein' named entity 'SDS-polyacrylamide gel electrophoresis' named entity 'membrane-bound' named entity 'protein' named entity 'positive and negative' named entity 'cytoplasmic' named entity 'cytoplasmic domain' named entity 'glycoproteins' named entity 'protein' named entity 'rough ER' named entity 'methionine' named entity 'murine' named entity 'exocytotic' named entity 'transmembrane domain' named entity 'antibodies' named entity 'protein' named entity 'serum' named entity 'oligosaccharides' named entity 'codon' named entity 'VSV' named entity 'cleavage' named entity 'protein' named entity 'protein' named entity 'rough ER' named entity 'radioiodination' named entity 'Golgi complex' named entity 'Immunoprecipitates' named entity 'glycoprotein' named entity 'extracellular' named entity 'coding sequences' named entity 'methionine' named entity 'cell line' named entity 'chimeric' named entity 'cytoplasmic domain' named entity 'SDS' named entity 'methionine' named entity 'cell surface' named entity 'glycoprotein' named entity 'cytoplasmic domain' named entity 'COS-1' named entity 'cytoplasmic domain' named entity 'protein' named entity 'wild type' named entity 'antibody' named entity 'protein' named entity 'VSV' named entity 'acetone'

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