About: Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections
Creator	Bertoletti, Antonio Chia, Adeline Banu, Nasirah Paravasivam, Komathi Garcia, Alfonso Gehring, Adam Grotenbreg, Gijsbert Ho, Zi
Source	Medline; PMC
abstract	Restoration of antigen-specific T cell immunity has the potential to clear persistent viral infection. T cell receptor (TCR) gene therapy can reconstitute CD8 T cell immunity in chronic patients. We cloned 10 virus-specific TCRs targeting 5 different viruses, causing chronic and acute infection. All 10 TCR genetic constructs were optimized for expression using a P2A sequence, codon optimization and the addition of a non-native disulfide bond. However, maximum TCR expression was only achieved after establishing the optimal orientation of the alpha and beta chains in the expression cassette; 9/10 TCRs favored the beta-P2A-alpha orientation over alpha-P2A-beta. Optimal TCR expression was associated with a significant increase in the frequency of IFN-gamma+ T cells. In addition, activating cells for transduction in the presence of Toll-like receptor ligands further enhanced IFN-gamma production. Thus, we have built a virus-specific TCR library that has potential for therapeutic intervention in chronic viral infection or virus-related cancers.
has issue date	2014-02-25(xsd:dateTime)
bibo:doi	10.1038/srep04166
bibo:pmid	24566718
has license	cc-by-nc-sa
sha1sum (hex)	62cd5be77a70a55d18199836987e521d4b992cbd
schema:url	https://doi.org/10.1038/srep04166
resource representing a document's title	Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections
has PubMed Central identifier	PMC3933865
has PubMed identifier	24566718
schema:publication	Sci Rep
resource representing a document's body	covid:62cd5be77a70a55d18199836987e521d4b992cbd#body_text
is schema:about of	named entity 'CANCERS' named entity 'SPECIFIC' named entity 'VIRUSES' named entity 'ACUTE INFECTION' named entity 'PRESENCE OF' named entity 'VIRUS' named entity 'LIBRARY' named entity 'SEQUENCE' named entity 'ASSOCIATED WITH' named entity 'ESTABLISHING' named entity 'POTENTIAL' named entity 'TARGETING' named entity 'GAMMA1' named entity 'NON-' named entity 'BETA' named entity 'SIGNIFICANT' named entity 'PATIENTS' named entity 'HAVE' named entity 'CHAINS' named entity 'P2A' named entity 'Restoration' named entity 'TCR' named entity 'cloned' named entity 'Viral' named entity 'Building' named entity 'BUILDING' named entity 'FREQUENCY' named entity 'T CELL RECEPTOR' named entity 'CLONED' named entity 'IFN' named entity 'CAUSING' named entity 'CD8' named entity 'DIFFERENT' named entity 'RESTORATION' named entity 'LIBRARY' named entity 'IMMUNOTHERAPY' named entity 'VIRUS' named entity 'TARGETED' named entity 'ORIENTATION' named entity 'INCREASE' named entity 'DISULFIDE BOND' named entity 'T CELL RECEPTOR' named entity 'TOLL-LIKE RECEPTOR' named entity 'NATIVE' named entity 'IFN-GAMMA PRODUCTION' named entity 'ACTIVATING' named entity 'LIGANDS' named entity 'TCR' named entity 'OPTIMAL' named entity '2F10' named entity 'T CELL IMMUNITY' named entity 'MAXIMUM' named entity 'T CELLS' named entity 'ANTIGEN' named entity 'VIRAL INFECTION' named entity 'CELLS' named entity 'SPECIFIC' named entity 'ALPHA' named entity 'THERAPEUTIC INTERVENTION' named entity 'CHRONIC' named entity 'CODON OPTIMIZATION' named entity 'VIRAL INFECTIONS' named entity 'GENETIC' named entity 'ENHANCED' named entity 'TRANSDUCTION' named entity 'PERSISTENT' named entity 'RECONSTITUTE' named entity 'GENE THERAPY' named entity 'EXPRESSION' named entity 'CASSETTE' named entity 'ADDITION' named entity 'USING' named entity 'RELATED' named entity 'CLEAR'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software