About: High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay
Creator	Miller, Aaron Bogdanovic, Elena D'aquila, Richard Demonbreun, Alexis Fallon, Katherine Mcdade Id, Thomas Mcnally, Elizabeth Mustanski, Brian Reiserid, Nina Vaughtid, Lauren Zelikovich, Aaron
Source	Medline; PMC
abstract	OBJECTIVE: Serological testing is needed to investigate the extent of transmission of SARS-CoV-2 from front-line essential workers to their household members. However, the requirement for serum/plasma limits serological testing to clinical settings where it is feasible to collect and process venous blood. To address this problem we developed a serological test for SARS-CoV-2 IgG antibodies that requires only a single drop of finger stick capillary whole blood, collected in the home and dried on filter paper (dried blood spot, DBS). We describe assay performance and demonstrate its utility for remote sampling with results from a community-based study. METHODS: An ELISA to the receptor binding domain of the SARS-CoV-2 spike protein was optimized to quantify IgG antibodies in DBS. Samples were self-collected from a community sample of 232 participants enriched with health care workers, including 30 known COVID-19 cases and their household members. RESULTS: Among 30 individuals sharing a household with a virus-confirmed case of COVID-19, 80% were seropositive. Of 202 community individuals without prior confirmed acute COVID-19 diagnoses, 36% were seropositive. Of documented convalescent COVID-19 cases from the community, 29 of 30 (97%) were seropositive for IgG antibodies to the receptor binding domain. CONCLUSION: DBS ELISA provides a minimally-invasive alternative to venous blood collection. Early analysis suggests a high rate of transmission among household members. High rates of seroconversion were also noted following recovery from infection. Serological testing for SARS-CoV-2 IgG antibodies in DBS samples can facilitate seroprevalence assessment in community settings to address epidemiological questions, monitor duration of antibody responses, and assess if antibodies against the spike protein correlate with protection from reinfection.
has issue date	2020-08-14(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0237833
bibo:pmid	32797108
has license	cc-by
sha1sum (hex)	62491f7e958d8dc3598141eeb236a0a793a2ba65
schema:url	https://doi.org/10.1371/journal.pone.0237833
resource representing a document's title	High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay
has PubMed Central identifier	PMC7428174
has PubMed identifier	32797108
schema:publication	PLoS One
resource representing a document's body	covid:62491f7e958d8dc3598141eeb236a0a793a2ba65#body_text
is schema:about of	named entity 'serum' named entity 'finger stick' named entity 'performance' named entity 'remote' named entity 'needed' named entity 'HOME' named entity 'SEROLOGICAL TEST' named entity 'DESCRIBE' named entity 'FEASIBLE' named entity 'PROCESS' named entity 'ANTIBODIES' named entity 'RESULTS' named entity 'TRANSMISSION' named entity 'BLOOD' named entity 'CAPILLARY' named entity 'SPOT' named entity 'transmission' named entity 'extent' named entity 'filter paper' named entity 'Serological' named entity 'SARS-CoV-2' named entity 'sampling' named entity 'finger stick' named entity 'SARS-CoV-2' named entity 'essential workers' named entity 'seroprevalence' named entity 'developed' named entity 'seropositive' named entity 'DBS' named entity 'serological' named entity 'seropositive' named entity 'Statistical analyses' named entity 'IgG' named entity 'DBS' named entity 'transmission rate' named entity 'seropositive' named entity 'PCR testing' named entity 'seropositive' named entity 'RBD' named entity 'seronegative' named entity 'community health' named entity 'finger stick' named entity 'serum samples' named entity 'DBS' named entity 'rhinorrhea' named entity 'health care workers' named entity 'spike protein' named entity 'seropositive' named entity 'tested positive' named entity 'PCR test' named entity 'DBS' named entity 'serum samples' named entity 'institutional review board' named entity 'seropositive' named entity 'seropositive' named entity 'seropositive' named entity 'minimally-invasive' named entity 'antigen' named entity 'non-invasive' named entity '28 days' named entity 'fatigue' named entity 'DBS' named entity 'DBS' named entity 'seronegative' named entity 'asymptomatic' named entity 'DBS' named entity 'IgG' named entity 'infection' named entity 'LLD' named entity 'spike protein' named entity 'seropositive'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software