About: Generation of Human CEACAM1 Transgenic Mice and Binding of Neisseria Opa Protein to Their Neutrophils

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About: Generation of Human CEACAM1 Transgenic Mice and Binding of Neisseria Opa Protein to Their Neutrophils Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generation of Human CEACAM1 Transgenic Mice and Binding of Neisseria Opa Protein to Their Neutrophils
Creator	Zhang, Nan Zhang, Zhifang Gu, Angel Tsark, Walter Shively, John
Source	PMC
abstract	BACKGROUND: Human CEACAM1 is a cell-cell adhesion molecule with multiple functions including insulin clearance in the liver, vasculogenesis in endothelial cells, lumen formation in the mammary gland, and binding of certain human pathogens. PRINCIPAL FINDINGS: Three genomic BAC clones containing the human CEACAM1 gene were microinjected into pronuclei of fertilized FVB mouse oocytes. The embryos were implanted in the oviducts of pseudopregnant females and allowed to develop to term. DNA from newborn mice was evaluated by PCR for the presence of the human CEACAM1 gene. Feces of the PCR positive offspring screened for expression of human CEACAM1. Using this assay, one out of five PCR positive lines was positive for human CEACAM1 expression and showed stable transmission to the F1 generation with the expected transmission frequency (0.5) for heterozygotes. Liver, lung, intestine, kidney, mammary gland, and prostate were strongly positive for the dual expression of both murine and human CEACAM1 and mimic that seen in human tissue. Peripheral blood and bone marrow granulocytes stained strongly for human CEACAM1 and bound Neisseria Opa proteins similar to that in human neutrophils. CONCLUSION: These transgenic animals may serve as a model for the binding of human pathogens to human CEACAM1.
has issue date	2010-04-09(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0010067
bibo:pmid	20404914
has license	cc-by
sha1sum (hex)	6204b15a79e3551e09c29cd4865fae0497462ec3
schema:url	https://doi.org/10.1371/journal.pone.0010067
resource representing a document's title	Generation of Human CEACAM1 Transgenic Mice and Binding of Neisseria Opa Protein to Their Neutrophils
has PubMed Central identifier	PMC2852402
has PubMed identifier	20404914
schema:publication	PLoS One
resource representing a document's body	covid:6204b15a79e3551e09c29cd4865fae0497462ec3#body_text
is schema:about of	named entity 'endothelial cells' named entity 'BINDING' named entity 'CELL-CELL ADHESION MOLECULE' named entity 'FUNCTIONS' named entity 'MULTIPLE' named entity 'CLEARANCE' named entity 'INCLUDING' named entity 'MAMMARY GLAND' named entity 'CEACAM1' named entity 'CERTAIN' named entity 'LIVER' named entity 'HUMAN' covid:arg/6204b15a79e3551e09c29cd4865fae0497462ec3 named entity 'THEIR' named entity 'PROTEIN ' named entity 'OPA' named entity 'CEACAM1' named entity 'NEUTROPHILS' named entity 'NEISSERIA' named entity 'BINDING' named entity 'A CELL' named entity 'PATHOGENS' named entity 'ENDOTHELIAL CELLS' named entity 'VASCULOGENESIS' named entity 'LUMEN FORMATION' named entity 'GENERATION' named entity 'TRANSGENIC MICE' named entity 'HUMAN' named entity 'IS A' named entity 'INSULIN' named entity 'BACKGROUND' named entity 'liver' named entity 'Human' named entity 'Neisseria' named entity 'lumen' named entity 'cell adhesion molecule' named entity 'vasculogenesis' named entity 'CEACAM1' named entity 'splice forms' named entity 'spleen' named entity 'murine' named entity 'CEACAM1' named entity 'CEACAM1' named entity 'CEACAM1' named entity 'neutrophils' named entity 'mRNA' named entity 'murine' named entity 'murine' named entity 'SHP1' named entity 'microinjections' named entity 'PCR analysis' named entity 'Cy5' named entity 'tyrosine phosphatases' named entity 'staining' named entity 'gene' named entity 'lung' named entity 'staining' named entity 'transgenic animals' named entity 'mounting medium' named entity 'bacteria' named entity 'negative control' named entity 'microinjection' named entity 'polyacrylamide' named entity 'CEACAM1' named entity 'CEACAM1' named entity 'reverse transcription' named entity 'neutrophil' named entity 'distal tubules' named entity 'positive control' named entity 'liver' named entity 'tissue staining' named entity 'CEACAM1' named entity 'chromosome 19' named entity 'protein' named entity 'GI tract' named entity 'epithelial cells' named entity 'liver'

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