About: Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

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About: Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China
Creator	Zhang, Shuang Wang, Xin Wang, Kai Dong, Hao Guo, Bing-Yan Hu, Xue-Gui Niu, Ting-Jiang Wang, Lei-Hua Yi, Shuai-Shu Zhao, Li-Yan
topic	covid:618dc6f68e1634ddb8d04360a157532372455da4#this
Source	Elsevier; Medline; PMC
abstract	Feline kobuvirus (FeKoV), a novel picornavirus of the genus kobuvirus, was initially identified in the feces of cats with diarrhea in South Korea in 2013. To date, there is only one report of the circulation of kobuvirus in cats in southern China. To investigate the presence and genetic variability of FeKoV in northeast China, 197 fecal samples were collected from 105 cats with obvious diarrhea and 92 asymptomatic cats in Shenyang, Jinzhou, Changchun, Jilin and Harbin regions, Northeast China, and viruses were detected by RT-PCR with universal primers targeting all kobuviruses. Kobuvirus was identified in 28 fecal samples with an overall prevalence of 14.2% (28/197) of which 20 samples were co-infected with feline parvovirus (FPV) and/or feline bocavirus (FBoV). Diarrhoeic cats had a higher kobuvirus prevalence (19.1%, 20/105) than asymptomatic cats (8.7%, 8/92). By genetic analysis based on partial 3D gene, all kobuvirus-positive samples were more closely related to previous FeKoV strains with high identities of 90.5%–97.8% and 96.6%–100% at the nucleotide and amino acid levels. Additionally, phylogenetic analysis based on the complete VP1 gene indicated that all FeKoV strains identified in this study were placed into a cluster, which separated from other reference strains previously reported, and three identical amino acid substitutions were present at the C-terminal of the VP1 protein for these FeKoV strains. Furthermore, two complete FeKoV polyprotein genomes were successfully obtained from two positive samples and designated 16JZ0605 and 17CC0811, respectively. The two strains shared 92.9%–94.9% nucleotide identities and 96.8%–98.4% amino acid identities to FeKoV prototype strains. Phylogenetic analysis indicated that FeKoVs were clustered according to their geographical regions, albeit with limited sequences support. This study provides the first molecular evidence that FeKoV circulates in cats in northeast China, and these FeKoVs exhibit genetic diversity and unique evolutionary trend.
has issue date	2018-12-06(xsd:dateTime)
bibo:doi	10.1016/j.meegid.2018.12.010
bibo:pmid	30529719
has license	no-cc
sha1sum (hex)	618dc6f68e1634ddb8d04360a157532372455da4
schema:url	https://doi.org/10.1016/j.meegid.2018.12.010
resource representing a document's title	Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China
has PubMed Central identifier	PMC7185515
has PubMed identifier	30529719
schema:publication	Infect Genet Evol
resource representing a document's body	covid:618dc6f68e1634ddb8d04360a157532372455da4#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/618dc6f68e1634ddb8d04360a157532372455da4
is schema:about of	named entity 'Shenyang' named entity 'Phylogenetic analysis' named entity '2013' named entity 'primers' named entity 'based' named entity 'based' named entity 'reference' named entity 'complete' named entity 'Northeast China' named entity 'prototype' covid:arg/618dc6f68e1634ddb8d04360a157532372455da4 named entity 'diarrhea' named entity 'samples' named entity 'partial' named entity 'study' named entity 'clustered' named entity 'support' named entity 'RT-PCR' named entity 'higher' named entity 'strains' named entity 'genomes' named entity 'initially' named entity 'identified' named entity 'high' named entity 'kobuvirus' named entity 'FPV' named entity 'gene' named entity 'VP1' named entity 'amino acid' named entity 'picornavirus' named entity 'co-infected' named entity 'nucleotide' named entity 'genus' named entity 'genomes' named entity 'Jilin' named entity 'asymptomatic' named entity 'phylogenetic analysis' named entity 'feces' named entity 'kobuvirus' named entity 'South Korea' named entity 'universal primers' named entity 'clones' named entity 'sheep' named entity 'VP3' named entity '3′UTR' named entity 'kobuvirus' named entity 'polyprotein' named entity 'genetics' named entity 'Plasmid' named entity 'VP1' named entity 'gene' named entity 'Invitrogen' named entity 'polyprotein' named entity 'Oem' named entity 'Kobuvirus' named entity 'reverse transcribed' named entity 'virus' named entity 'Shenyang' named entity 'positive-sense RNA genome' named entity 'proline' named entity 'gene' named entity 'gene' named entity 'VP1' named entity 'gene' named entity 'amino acid' named entity 'polyproline helix' named entity 'amino acid' named entity 'polyprotein' named entity 'polyprotein' named entity 'Phylogenetic analyses'

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