About: Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro

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About: Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro
Creator	Kim, Meehyein Kim, Baek Oh, Hyeryeon Ro, Hyun-Joo Go, Yun
Source	Medline; PMC
abstract	Dengue fever is one of the most important mosquito-borne viral infections in large parts of tropical and subtropical countries and is a significant public health concern and socioeconomic burden. There is an urgent need to develop antivirals that can effectively reduce dengue virus (DENV) replication and decrease viral load. Niclosamide, an antiparasitic drug approved for human use, has been recently identified as an effective antiviral agent against a number of pH-dependent viruses, including flaviviruses. Here, we reveal that neutralization of low-pH intracellular compartments by niclosamide affects multiple steps of the DENV infectious cycle. Specifically, niclosamide-induced endosomal neutralization not only prevents viral RNA replication but also affects the maturation of DENV particles, rendering them non-infectious. We found that niclosamide-induced endosomal neutralization prevented E glycoprotein conformational changes on the virion surface of flaviviruses, resulting in the release of non-infectious immature virus particles with uncleaved pr peptide from host cells. Collectively, our findings support the potential application of niclosamide as an antiviral agent against flavivirus infection and highlight a previously uncharacterized mechanism of action of the drug.
has issue date	2019-06-18(xsd:dateTime)
bibo:doi	10.1038/s41598-019-45095-1
bibo:pmid	31213630
has license	cc-by
sha1sum (hex)	61722c462b054f36461375e96e502cbf22648c04
schema:url	https://doi.org/10.1038/s41598-019-45095-1
resource representing a document's title	Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro
has PubMed Central identifier	PMC6582152
has PubMed identifier	31213630
schema:publication	Sci Rep
resource representing a document's body	covid:61722c462b054f36461375e96e502cbf22648c04#body_text
is schema:about of	named entity 'infection' named entity 'application' named entity 'effectively' named entity 'maturation' covid:arg/61722c462b054f36461375e96e502cbf22648c04 named entity 'Niclosamide' named entity 'viral' named entity 'identified' named entity 'socioeconomic' named entity 'highlight' named entity 'DENV' named entity 'host' named entity 'intracellular compartments' named entity 'surface' named entity 'endosomal' named entity 'niclosamide' named entity 'viral load' named entity 'subtropical' named entity 'flavivirus' named entity 'non-infectious' named entity 'endosomal' named entity 'host cells' named entity 'infection' named entity 'cycle' named entity 'plaque assay' named entity 'standard curve' named entity 'cytotoxic effects' named entity 'genome' named entity 'infection' named entity 'DMSO' named entity 'FACS' named entity 'virions' named entity 'MTT' named entity 'transmission electron microscopy' named entity 'extracellular' named entity 'ZIKV' named entity 'viral RNA replication' named entity 'centrifugation' named entity 'DENV' named entity 'virus' named entity 'niclosamide' named entity 'Image analysis' named entity 'virus' named entity 'NS3' named entity 'endosomal' named entity 'niclosamide' named entity 'dose-dependent manner' named entity 'Carl Zeiss' named entity 'niclosamide' named entity 'infection' named entity 'Huh-7' named entity 'FACS' named entity 'viral protein' named entity 'gene' named entity 'BHK-21 cells' named entity 'serotypes' named entity 'low pH' named entity 'endosomal' named entity 'membrane fusion' named entity 'U937' named entity 'viral RNA replication' named entity 'niclosamide' named entity 'viral life cycle' named entity '3-log reduction' named entity 'Tecan' named entity 'Huh-7' named entity 'ZIKV' named entity 'niclosamide' named entity 'Huh-7' named entity 'infection' named entity 'plasmid' named entity 'dsRNA' named entity 'virion' named entity 'infection' named entity 'niclosamide' named entity 'viral replication' named entity 'Bafilomycin A1'

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