About: Gain‐of‐function experiments with bacteriophage lambda uncover residues under diversifying selection in nature

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Gain‐of‐function experiments with bacteriophage lambda uncover residues under diversifying selection in nature
Creator	Johnson, Daniel Maddamsetti, Rohan Marks, Debora Meyer, Justin Petrie, Katherine Spielman, Stephanie
Source	Medline; PMC
abstract	Viral gain‐of‐function mutations frequently evolve during laboratory experiments. Whether the specific mutations that evolve in the lab also evolve in nature and whether they have the same impact on evolution in the real world is unknown. We studied a model virus, bacteriophage λ, that repeatedly evolves to exploit a new host receptor under typical laboratory conditions. Here, we demonstrate that two residues of λ’s J protein are required for the new function. In natural λ variants, these amino acid sites are highly diverse and evolve at high rates. Insertions and deletions at these locations are associated with phylogenetic patterns indicative of ecological diversification. Our results show that viral evolution in the laboratory mirrors that in nature and that laboratory experiments can be coupled with protein sequence analyses to identify the causes of viral evolution in the real world. Furthermore, our results provide evidence for widespread host‐shift evolution in lambdoid viruses.
has issue date	2018-09-11(xsd:dateTime)
bibo:doi	10.1111/evo.13586
bibo:pmid	30152871
has license	cc-by-nc-nd
sha1sum (hex)	60f1455bed921da2e4f3df7aae854cb8e142ff44
schema:url	https://doi.org/10.1111/evo.13586
resource representing a document's title	Gain‐of‐function experiments with bacteriophage lambda uncover residues under diversifying selection in nature
has PubMed Central identifier	PMC6646904
has PubMed identifier	30152871
schema:publication	Evolution
resource representing a document's body	covid:60f1455bed921da2e4f3df7aae854cb8e142ff44#body_text
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