About: A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification
Creator	Nasheuer,
Source	PMC
abstract	In principle, PCR generates large quantities of DNA from a minute amount of nucleic acid starting material using a methodology similar to (but much simpler than) that seen in living cells. For living cells, in vivo DNA synthesis is dependent upon a well defined but complex set of enzymes and co-factors, which have evolved to act in a concerted fashion during the synthetic phase (S-phase) of the cell cycle. In comparison, PCR facilitates in vitro DNA synthesis in a much simpler fashion, making use of a smaller set of defined ingredients and reaction conditions involving relatively high temperatures. The range of factors contributing to successful PCR amplification is reviewed below.
has issue date	2008-01-01(xsd:dateTime)
bibo:doi	10.1007/978-1-4020-6241-4_2
has license	no-cc
sha1sum (hex)	60dae494077004ff268f3085bde38efde30dee27
schema:url	https://doi.org/10.1007/978-1-4020-6241-4_2
resource representing a document's title	A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification
has PubMed Central identifier	PMC7120002
schema:publication	Principles and Technical Aspects of PCR Amplification
resource representing a document's body	covid:60dae494077004ff268f3085bde38efde30dee27#body_text
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