About: Determination of microsomal lauric acid hydroxylase activity by HPLC with flow-through radiochemical quantitation

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About: Determination of microsomal lauric acid hydroxylase activity by HPLC with flow-through radiochemical quantitation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Determination of microsomal lauric acid hydroxylase activity by HPLC with flow-through radiochemical quantitation
Creator	Eckardt, John Lee, Ann Newton, Romano, Maria Straub, Kenneth Yodis, Regina
Source	Elsevier; Medline; PMC
abstract	Abstract An assay for the microsomal hydroxylation of lauric acid (LA), based on HPLC with flow-through radiochemical detection, has been developed. Conditions were optimized for resolution and quantitation of three microsomal metabolites of LA, one of which has not been reported previously as a metabolite of LA in mammalian microsomal incubations. These products, 12-(ω)-hydroxy-LA, 11-(ω-1)-hydroxy-LA, and a novel metabolite, 10-(ω-2)-hydroxy-LA, were isolated by HPLC and identified by gas chromatography/mass spectrometry. In the presence of NADPH, the formation of all three metabolites was linear with time and microsomal protein concentration. Hydrogen peroxide also supported the microsomal metabolism of LA, although the ratio of metabolites was substantially different than that produced by NADPH-supported microsomes. Several biochemical probes (metyrapone, α-naphthoflavone, 2-diethylaminoethyl-2,2-diphenylvalerate hydrochloride, and 10-undecynoic acid) were used to dissociate the three LA hydroxylase activities. These experiments suggest that the site-specific hydroxylation [ω-, (ω-1)-, (ω-2)-] of LA may be catalyzed by different isozymes of cytochrome P-450.
has issue date	1988-04-30(xsd:dateTime)
bibo:doi	10.1016/0003-2697(88)90093-0
bibo:pmid	3389520
has license	els-covid
sha1sum (hex)	60bf634cf15582a6b7f72eb3811ca5d8b8825c44
schema:url	https://doi.org/10.1016/0003-2697%2888%2990093-0
resource representing a document's title	Determination of microsomal lauric acid hydroxylase activity by HPLC with flow-through radiochemical quantitation
has PubMed Central identifier	PMC7119439
has PubMed identifier	3389520
schema:publication	Analytical Biochemistry
resource representing a document's body	covid:60bf634cf15582a6b7f72eb3811ca5d8b8825c44#body_text
is schema:about of	named entity 'detection' named entity 'microsomal' named entity 'radiochemical' named entity 'acid' named entity 'hydroxylase' named entity 'microsomal' named entity 'Acid' named entity 'HPLC' named entity 'MICROSOMAL' named entity 'NOVEL' named entity 'ACID' named entity 'NAPHTHOFLAVONE' named entity 'RATIO' named entity 'DIFFERENT' named entity 'METABOLITE' named entity 'PRODUCTS' named entity 'USED' named entity 'MICROSOMES' named entity 'PRESENCE OF' named entity 'EXPERIMENTS' named entity 'ACTIVITIES' named entity 'HYDROXYLASE' named entity 'ASSAY' named entity 'IDENTIFIED BY' named entity 'MAMMALIAN' named entity 'RADIOCHEMICAL' named entity 'HYDROXYLASE ACTIVITY' named entity 'ISOZYMES' named entity 'MAY BE' named entity 'REPORTED' named entity 'LINEAR' named entity 'CONDITIONS' named entity 'SPECIFIC' named entity 'CYTOCHROME P-450' named entity 'BIOCHEMICAL' named entity 'SITE' named entity 'THESE' named entity 'LAURIC ACID' named entity 'PROBES' named entity 'HYDROXY' named entity 'QUANTITATION' named entity 'RADIOCHEMICAL' named entity 'DETERMINATION' named entity 'ACID' named entity 'SUGGEST' named entity 'MICROSOMAL' named entity 'QUANTITATION' named entity 'FORMATION' named entity 'ONE OF' named entity 'WITH TIME' named entity 'RESOLUTION' named entity 'PROTEIN CONCENTRATION' named entity 'PREVIOUSLY' named entity 'GAS CHROMATOGRAPHY' named entity 'BASED' named entity 'METABOLITES' named entity 'ISOLATED' named entity 'HYDROGEN PEROXIDE' named entity 'HYDROCHLORIDE' named entity 'SUPPORTED' named entity 'FLOW' named entity 'HYDROXYLATION' named entity 'NADPH' named entity 'SPECTROMETRY' named entity 'HPLC' named entity 'DETECTION' covid:arg/60bf634cf15582a6b7f72eb3811ca5d8b8825c44 named entity 'activities' named entity 'products' named entity 'quantitation' named entity 'based' named entity 'microsomes' named entity 'metabolism' named entity 'hydrochloride' named entity 'metabolites' named entity 'cytochrome P-450'

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