About: Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices

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About: Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices
Creator	Kralik, Petr Mikel, Pavel Vasickova, Petra Kulich, Pavel Malenovska, Hana Tesarik, Radek Vesely, Tomas Canada, Agri-Food Cz, Jones, Tineke Sandra, Canada
Source	PMC
abstract	The detection and quantification of enteric RNA viruses is based on isolation of viral RNA from the sample followed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). To control the whole process of analysis and in order to guarantee the validity and reliability of results, process control viruses (PCV) are used. The present article describes the process of preparation and use of such PCV– MS2 phage-like particles (MS2 PLP) – in RT-qPCR detection and quantification of enteric RNA viruses. The MS2 PLP were derived from bacteriophage MS2 carrying a unique and specific de novo-constructed RNA target sequence originating from the DNA of two extinct species. The amount of prepared MS2 particles was quantified using four independent methods – UV spectrophotometry, fluorimetry, transmission electron microscopy and a specifically developed duplex RT-qPCR. To evaluate the usefulness of MS2 PLP in routine diagnostics different matrices known to harbor enteric RNA viruses (swab samples, liver tissue, serum, feces, and vegetables) were artificially contaminated with specific amounts of MS2 PLP. The extraction efficiencies were calculated for each individual matrix. The prepared particles fulfill all requirements for PCV – they are very stable, non-infectious, and are genetically distinct from the target RNA viruses. Due to these properties they represent a good morphological and physiochemical model. The use of MS2 PLP as a PCV in detection and quantification of enteric RNA viruses was evaluated in different types of matrices.
has issue date	2016-12-01(xsd:dateTime)
bibo:doi	10.3389/fmicb.2016.01911
bibo:pmid	28133456
has license	cc-by
sha1sum (hex)	5feab9a11d87a3bcb88dedd98948063b7d259ae2
schema:url	https://doi.org/10.3389/fmicb.2016.01911
resource representing a document's title	Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices
has PubMed Central identifier	PMC5234545
has PubMed identifier	28133456
schema:publication	Front Microbiol
resource representing a document's body	covid:5feab9a11d87a3bcb88dedd98948063b7d259ae2#body_text
is schema:about of	named entity 'THEIR' named entity 'DETECTION' named entity 'viruses' named entity 'tissue' named entity 'matrices' named entity 'liver' named entity 'evaluated' named entity 'RNA' named entity 'bacteriophage MS2' named entity 'detection' named entity 'LIKE' named entity 'VIRUSES' named entity 'QUANTITATIVE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION' named entity 'RNA VIRUSES' named entity 'GOOD' named entity 'VEGETABLES' named entity 'DUPLEX' named entity 'TO CONTROL' named entity 'PROPERTIES' named entity 'DETECTION' named entity 'PROCESS' named entity 'PCV' named entity 'FECES' named entity 'USEFULNESS' named entity 'PREPARED' named entity 'LIKE' named entity 'MODEL' named entity 'RESULTS' named entity 'REQUIREMENTS' named entity 'RNA' named entity 'HARBOR' named entity 'ORDER' named entity 'ENTERIC' named entity 'VIRAL RNA' named entity 'QPCR' named entity 'STABLE' named entity 'INFECTIOUS' named entity 'METHODS' named entity 'NON-' named entity 'QUANTIFICATION' named entity 'DNA' named entity 'LIVER TISSUE' named entity 'VALIDITY' named entity 'ROUTINE' named entity 'USE OF' named entity 'MATRICES' named entity 'BASED' named entity 'PRESENT' named entity 'REPRESENT' named entity 'MORPHOLOGICAL' named entity 'MATRIX' named entity 'MS2 PHAGE' named entity 'EXTINCT SPECIES' named entity 'TRANSMISSION ELECTRON MICROSCOPY' named entity 'RNA VIRUSES' named entity 'ENTERIC' named entity 'PHAGE' named entity 'TARGET' named entity 'PREPARATION' named entity 'FOLLOWED BY' named entity 'RELIABILITY' named entity 'INDIVIDUAL' named entity 'RT-QPCR' named entity 'USED' named entity 'CARRYING' named entity 'THESE' named entity 'INDEPENDENT' named entity 'CONTAMINATED' named entity 'QUANTIFICATION' named entity 'EFFICIENCIES' named entity 'CONTROL'

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