About: Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family

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About: Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family
Creator	Muhammad, & Shah, Ali Habib, Mudasser Hemmatzadeh, Farhid Iqbal, Mazhar Majid, & Muddassar, Muhammad Rahman, Moazur Shah, Salahuddin Shehzad, Aamir Tariq, Anam Zafar, Maryam
Source	PMC
abstract	Birnaviridae is a family of viruses (birnaviruses) which consists of four genera, members of which cause diseases in fish, birds, mollusks, and insects. The genome of birnaviruses encodes the highly immunogenic VP2 capsid protein. In order to demonstrate that the VP2 protein can be exploited as a diagnostic antigen for birnaviruses, we developed a lateral flow assay based on the surface-exposed VP2 protrusion domain of a representative birnavirus, infectious bursal disease virus (IBDV) of serotype 1 which causes the highly devastating infectious bursal disease in chickens. The biophysical characterization of the purified domain reveals that the domain predominantly consists of β-sheets, exists in a trimeric form, and remains folded at high temperatures, making it suitable for diagnostic purposes. Owing to its highly immunogenic nature and excellent biophysical properties, we employed the VP2 protrusion domain in a gold nanoparticle-based lateral flow assay for rapid detection of anti-IBDV antibodies in serum samples of infected chickens. Our results indicate that the domain binds anti-IBDV antibodies with high specificity during laboratory testing and on-site testing. The lateral flow assay reported here yields comparable results in a qualitative manner as obtained through a commercial enzyme-linked immunosorbent assay (ELISA). As VP2 is a common capsid protein of birnaviruses, the lateral flow assay can be generalized for other birnaviruses, and members of Tetraviridae and Nodaviridae families which contain homologous VP2 capsid proteins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00253-020-10458-6) contains supplementary material, which is available to authorized users.
has issue date	2020-02-23(xsd:dateTime)
bibo:doi	10.1007/s00253-020-10458-6
bibo:pmid	32088761
has license	no-cc
sha1sum (hex)	5f94038812d8ce96fe68e407e720a15a747560a5
schema:url	https://doi.org/10.1007/s00253-020-10458-6
resource representing a document's title	Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family
has PubMed Central identifier	PMC7222154
has PubMed identifier	32088761
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:5f94038812d8ce96fe68e407e720a15a747560a5#body_text
is schema:about of	named entity 'protein' named entity 'domain' named entity 'domain' named entity 'qualitative' named entity 'capsid' named entity 'generalized' named entity 'developed' named entity 'VIRUSES' named entity 'FLOW' named entity 'BINDS' named entity 'REVEALS' named entity 'protein' named entity 'chickens' named entity 'viruses' named entity 'capsid' named entity 'immunogenic' named entity 'chickens' named entity 'lateral' named entity 'antibodies' named entity 'domain' named entity 'structural studies' named entity 'colloidal gold' named entity 'homologous' named entity 'lateral flow assays' named entity 'antigen' named entity 'Antigenic' named entity 'molecular weight' named entity 'genera' named entity 'exists' named entity 'Nodaviridae' named entity 'capsid' named entity 'biophysical' named entity 'flow' named entity 'VP2' named entity 'VP2' named entity 'serotype' named entity 'birnaviruses' named entity 'biophysical properties' named entity 'birnaviruses' named entity 'birnaviruses' named entity 'capsid protein' named entity 'capsid proteins' named entity 'antigen' named entity 'birds' named entity 'demonstrate' named entity 'Thermo Scientific' named entity 'eukaryotic' named entity 'enzootic' named entity 'E. coli' named entity 'birnavirus' named entity 'Pakistan' named entity 'viruses' named entity '220 nm' named entity 'gel filtration' named entity 'antibodies' named entity 'protein' named entity 'capsid protein' named entity 'absorbance' named entity 'FTIR' named entity 'Virology' named entity 'sodium phosphate' named entity 'immune responses' named entity 'kDa' named entity 'crystal structure' named entity 'antibodies' named entity 'immunoassays' named entity 'protein' named entity 'IBDV' named entity 'w/v' named entity 'diagnostic purposes'

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