About: Two Different Conformations in Hepatitis C Virus p7 Protein Account for Proton Transport and Dye Release

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Two Different Conformations in Hepatitis C Virus p7 Protein Account for Proton Transport and Dye Release
Creator	Torres, J Gan, S Surya, W Vararattanavech, A
Source	Medline; PMC
abstract	The p7 protein from the hepatitis C virus (HCV) is a 63 amino acid long polypeptide that is essential for replication, and is involved in protein trafficking and proton transport. Therefore, p7 is a possible target for antivirals. The consensus model for the channel formed by p7 protein is a hexameric or heptameric oligomer of α-helical hairpin monomers, each having two transmembrane domains, TM1 and TM2, where the N-terminal TM1 would face the lumen of this channel. A reported high-throughput functional assay to search for p7 channel inhibitors is based on carboxyfluorescein (CF) release from liposomes after p7 addition. However, the rationale for the dual ability of p7 to serve as an ion or proton channel in the infected cell, and to permeabilize membranes to large molecules like CF is not clear. We have recreated both activities in vitro, examining the conformation present in these assays using infrared spectroscopy. Our results indicate that an α-helical form of p7, which can transport protons, is not able to elicit CF release. In contrast, membrane permeabilization to CF is observed when p7 contains a high percentage of β-structure, or when using a C-terminal fragment of p7, encompassing TM2. We propose that the reported inhibitory effect of some small compounds, e.g., rimantadine, on both CF release and proton transport can be explained via binding to the membrane-inserted C-terminal half of p7, increasing its rigidity, in a similar way to the influenza A M2-rimantadine interaction.
has issue date	2014-01-07(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0078494
bibo:pmid	24409277
has license	cc-by
sha1sum (hex)	5e8f40e1096456d1b5b0c24b57104bf15f45d37e
schema:url	https://doi.org/10.1371/journal.pone.0078494
resource representing a document's title	Two Different Conformations in Hepatitis C Virus p7 Protein Account for Proton Transport and Dye Release
has PubMed Central identifier	PMC3883635
has PubMed identifier	24409277
schema:publication	PLoS One
resource representing a document's body	covid:5e8f40e1096456d1b5b0c24b57104bf15f45d37e#body_text
is schema:about of	named entity 'conformation' named entity 'target' named entity 'membrane' named entity 'transmembrane domains' named entity 'p7 protein' named entity 'involved' named entity 'C-terminal' named entity 'hepatitis C virus' named entity 'inhibitors' named entity 'Proton' named entity 'Transport' named entity 'HELICAL' named entity 'ESSENTIAL' named entity 'RELEASE' named entity 'TO SERVE AS' named entity 'FORMED' named entity 'REPORTED' named entity 'IN VITRO' named entity 'INVOLVED' named entity 'PROTON TRANSPORT' named entity 'SEARCH' named entity 'CONFORMATION' named entity 'RELEASE' named entity 'SIMILAR' named entity 'USING' named entity 'MEMBRANES' named entity 'ION' named entity 'MEMBRANE' named entity 'WHERE' named entity 'INHIBITORY EFFECT' covid:arg/5e8f40e1096456d1b5b0c24b57104bf15f45d37e named entity 'ACCOUNT' named entity 'LIKE' named entity 'SMALL' named entity 'RESULTS' named entity 'INTERACTION' named entity 'TM1' named entity 'POSSIBLE' named entity 'PROTONS' named entity 'C-TERMINAL' named entity 'EXAMINING' named entity 'EXPLAINED' named entity 'DIFFERENT' named entity 'THESE' named entity 'POLYPEPTIDE' named entity 'FORM' named entity 'TRANSPORT' named entity 'ADDITION' named entity 'HEPATITIS C VIRUS' named entity 'BINDING' named entity 'LIPOSOMES' named entity 'OBSERVED' named entity 'TARGET' named entity 'LARGE' named entity 'PRESENT' named entity 'LUMEN' named entity 'HALF' named entity 'OLIGOMER' named entity 'PROTEIN TRAFFICKING' named entity 'TM2' named entity 'FUNCTIONAL ASSAY' named entity 'MONOMERS' named entity 'TRANSMEMBRANE' named entity 'LONG' named entity 'MODEL' named entity 'RATIONALE' named entity 'CLEAR' named entity 'INCREASING' named entity 'CONTRAST' named entity 'HIGH PERCENTAGE' named entity 'CONTAINS' named entity 'CONSENSUS' named entity 'FRAGMENT OF' named entity 'FACE' named entity 'RIMANTADINE' named entity 'BASED' named entity 'PROTEIN ' named entity 'CARBOXYFLUORESCEIN'

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