About: Yeast surface display-based microfluidic immunoassay

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Yeast surface display-based microfluidic immunoassay
Creator	Wang, Jing Hsing, I-Ming Cheng, Danhui Luo, Xiaoteng Wu, Hongkai Chan, Kwok-Lun, Jay
Source	Elsevier; Medline; PMC
abstract	Abstract In this paper, we present a new microfluidic immunoassay platform, which is based on the synergistic combination of the yeast surface display (YSD) technique and the microfluidic technology. Utilizing the YSD technique, antigens specific to the target antibody are displayed on the surface of engineered yeast cells with intracellular fluorescent proteins. The displayed antigens are then used for the detection of the target antibody, with the yeast cells as fluorescent labels. Multiplex immunoassay can be readily realized by using yeast cells expressing different intracellular fluorescent proteins to display different antigens. The implementation of this YSD-based immunoassay on the microfluidic platform eliminates the need for the bulky, complex and expensive flow cytometer. To improve the detection sensitivity and to eliminate the need for pumping, a functionalized micro pillar array (MPA) is incorporated in the microfluidic chip, resulting in a detection limit of 5ng/mL (or 1ng in terms of amount) and enhanced compatibility with practical applications such as clinical biopsy. This new platform has a high potential to be integrated into microfluidic detection systems to enable portable diagnostics in the future.
has issue date	2012-05-20(xsd:dateTime)
bibo:doi	10.1016/j.snb.2012.02.048
bibo:pmid	32288242
has license	els-covid
sha1sum (hex)	5d68a06a89d1de979b9c8f49e054b79490081b85
schema:url	https://doi.org/10.1016/j.snb.2012.02.048
resource representing a document's title	Yeast surface display-based microfluidic immunoassay
has PubMed Central identifier	PMC7127699
has PubMed identifier	32288242
schema:publication	Sensors and Actuators B: Chemical
resource representing a document's body	covid:5d68a06a89d1de979b9c8f49e054b79490081b85#body_text
is schema:about of	named entity 'YEAST' named entity 'antigens' named entity 'synergistic' named entity 'Multiplex' named entity 'microfluidic' named entity 'USED' named entity 'MICRO' named entity 'PROTEINS' named entity 'SENSITIVITY' named entity 'SURFACE DISPLAY' named entity 'COMPLEX' named entity 'MICROFLUIDIC' named entity 'SYSTEMS' named entity 'PORTABLE' named entity 'FLOW CYTOMETER' named entity 'NEW' named entity 'FUTURE' named entity 'DISPLAYED' named entity 'ENABLE' named entity 'ANTIGENS' named entity 'DETECTION' named entity 'TECHNOLOGY' named entity 'PRESENT' named entity 'DIFFERENT' named entity 'MICROFLUIDIC' named entity 'USING' covid:arg/5d68a06a89d1de979b9c8f49e054b79490081b85 named entity 'ENHANCED' named entity 'PAPER' named entity 'SURFACE' named entity 'BASED' named entity 'COMPATIBILITY' named entity 'NEED FOR' named entity 'PILLAR' named entity 'DIAGNOSTICS' named entity 'PLATFORM' named entity 'SPECIFIC' named entity 'ENGINEERED' named entity 'BASED' named entity 'APPLICATIONS' named entity 'SURFACE DISPLAY' named entity 'IMPLEMENTATION' named entity 'TO DISPLAY' named entity 'ANTIBODY' named entity 'LABELS' named entity 'COMBINATION' named entity 'INTRACELLULAR' named entity 'CHIP' named entity 'PUMPING' named entity 'DETECTION LIMIT' named entity 'CLINICAL' named entity 'BIOPSY' named entity 'SYNERGISTIC' named entity 'HIGH' named entity 'IMMUNOASSAY' named entity 'TECHNIQUE' named entity 'INTEGRATED' named entity 'IMMUNOASSAY' named entity 'ARRAY' named entity 'TARGET' named entity 'BULKY' named entity 'FLUORESCENT' named entity 'YEAST' named entity 'POTENTIAL' named entity 'TERMS' named entity 'MULTIPLEX' named entity 'RESULTING IN' named entity 'functionalized' named entity 'antigens' named entity 'realized' named entity 'immunoassay' named entity 'fluorescent proteins' named entity 'bulky' named entity 'displayed' named entity 'expressing'

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