About: Generation and characterization of bat-induced pluripotent stem cells

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About: Generation and characterization of bat-induced pluripotent stem cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Generation and characterization of bat-induced pluripotent stem cells
Creator	Li, Ning Mo, Xiaohui Wu, Sen
source	Elsevier; Medline; PMC
abstract	Abstract Induced pluripotent stem cells (iPSCs) were first generated from mouse embryonic fibroblasts in the year 2006. These cells resemble the typical morphology of embryonic stem cells, express pluripotency markers, and are able to transmit through germlines. To date, iPSCs of many species have been generated, whereas generation of bat iPSCs (biPSCs) has not been reported. To facilitate in-depth study of bats at the molecular and cellular levels, we describe the successful derivation of biPSCs with a piggyBac (PB) vector that contains eight reprogramming factors Oct4, Sox2, Klf4, Nanog, cMyc, Lin28, Nr5a2, and miR302/367. These biPSCs were cultured in media containing leukemia inhibitory factor and three small molecule inhibitors (CHIR99021, PD0325901, and A8301). They retained normal karyotype, displayed alkaline phosphatase activity, and expressed pluripotency markers Oct4, Sox2, Nanog, TBX3, and TRA-1-60. They could differentiate in vitro to form embryoid bodies and in vivo to form teratomas that contained tissue cells of all three germ layers. Generation of biPSCs will facilitate future studies on the mechanisms of antiviral immunity and longevity of bats at the cellular level.
has issue date	2014-07-15(xsd:dateTime)
bibo:doi	10.1016/j.theriogenology.2014.04.001
bibo:pmid	24853281
has license	els-covid
sha1sum (hex)	5d56a6c78172633f889470e3139040fb17b9cf8a
schema:url	https://doi.org/10.1016/j.theriogenology.2014.04.001
resource representing a document's title	Generation and characterization of bat-induced pluripotent stem cells
has PubMed Central identifier	PMC7103130
has PubMed identifier	24853281
schema:publication	Theriogenology
resource representing a document's body	covid:5d56a6c78172633f889470e3139040fb17b9cf8a#body_text
is schema:about of	named entity 'embryonic' named entity 'future studies' named entity 'generated' named entity 'YEAR' named entity 'FIBROBLASTS' named entity 'LEVELS' named entity 'DESCRIBE' named entity 'DISPLAYED' named entity 'MARKERS' named entity 'BAT' named entity 'PIGGYBAC' named entity 'IN VITRO' named entity 'DERIVATION' named entity 'EMBRYONIC STEM CELLS' named entity 'CONTAINS' named entity 'TBX3' named entity 'HAVE' named entity 'DIFFERENTIATE' named entity 'EXPRESSED' named entity 'TRA-1' named entity 'LEVEL' named entity 'VECTOR' named entity 'MEDIA' named entity 'SUCCESSFUL' named entity 'OCT4' named entity 'REPROGRAMMING' covid:arg/5d56a6c78172633f889470e3139040fb17b9cf8a named entity 'BATS' named entity 'RETAINED' named entity 'MOLECULAR' named entity 'IPSCS' named entity 'THESE' named entity 'SMALL MOLECULE' named entity 'MORPHOLOGY' named entity 'NORMAL KARYOTYPE' named entity 'CONTAINING' named entity 'NANOG' named entity 'SOX2' named entity 'LEUKEMIA INHIBITORY FACTOR' named entity 'EMBRYONIC' named entity 'EMBRYOID BODIES' named entity 'GENERATED' named entity 'CHARACTERIZATION' named entity 'CULTURED' named entity 'INDUCED PLURIPOTENT STEM CELLS' named entity 'GENERATION' named entity 'BAT' named entity 'NR5A2' named entity 'CELLS' named entity 'TISSUE' named entity 'ANTIVIRAL' named entity 'STUDIES' named entity 'TERATOMAS' named entity 'TO TRANSMIT' named entity 'DATE' named entity 'IN VIVO' named entity 'INDUCED PLURIPOTENT STEM CELLS' named entity 'IMMUNITY' named entity 'MECHANISMS' named entity 'INHIBITORS' named entity 'TO FORM' named entity 'GENERATION' named entity 'FUTURE' named entity 'KLF4' named entity 'PD0325901' named entity 'PLURIPOTENCY' named entity 'GERM LAYERS' named entity 'ALKALINE PHOSPHATASE ACTIVITY' named entity 'MOUSE' named entity 'TYPICAL' named entity 'SPECIES' named entity 'CELLULAR' named entity 'STUDY' named entity 'REPORTED' named entity 'DEPTH' named entity 'EXPRESS' named entity 'LIN28' named entity 'LONGEVITY' named entity 'CMYC'

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