About: Direct on-the-spot detection of SARS-CoV-2 in patients

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About: Direct on-the-spot detection of SARS-CoV-2 in patients Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Direct on-the-spot detection of SARS-CoV-2 in patients
Creator	Szwarcwort-Cohen, Moran King, Daniel Paul, Mical Ben-Assa, Nadav Capucha, Tal Chowers, Michal Elbaum, Lilach Gefen, Tal Geva-Zatorsky, Naama Hajjo, Haitham Kaplan, Shai Mandelbaum, Noa Naddaf, Rawi Rogov, Peter Rotem, Assaf
Source	Medline; PMC
abstract	Many countries are currently in a state of lockdown due to the SARS-CoV-2 pandemic. One key requirement to safely transition out of lockdown is the continuous testing of the population to identify infected subjects. Currently, detection is performed at points of care using quantitative reverse-transcription PCR, thus requiring dedicated professionals and equipment. Here, we developed a protocol based on reverse transcribed loop-mediated isothermal amplification for the detection of SARS-CoV-2. This protocol is applied directly to SARS-CoV-2 nose and throat swabs, with no RNA purification step required. We tested this protocol on over 180 suspected patients, and compared the results to those obtained using the standard method. We further succeeded in applying the protocol to self-collected saliva samples from confirmed cases. Since the proposed protocol can detect SARS-CoV-2 from saliva and provides on-the-spot results, it allows simple and continuous surveillance of the community. IMPACT STATEMENT: Humanity is currently experiencing a global pandemic with devastating implications on human health and the economy. Most countries are gradually exiting their lockdown state. We are currently lacking rapid and simple viral detections, especially methods that can be performed in the household. Here, we applied RT-LAMP directly on human clinical swabs and self-collected saliva samples. We adjusted the method to allow simple and rapid viral detection, with no RNA purification steps. By testing our method on over 180 human samples, we determined its sensitivity, and by applying it to other viruses, we determined its specificity. We believe this method has a promising potential to be applied world-wide as a simple and cheap surveillance test for SARS-CoV-2.
has issue date	2020-07-16(xsd:dateTime)
bibo:doi	10.1177/1535370220941819
bibo:pmid	32668983
has license	cc-by
sha1sum (hex)	5ce97aacf236a9cbac4393e43c83488effb9b191
schema:url	https://doi.org/10.1177/1535370220941819
resource representing a document's title	Direct on-the-spot detection of SARS-CoV-2 in patients
has PubMed Central identifier	PMC7385438
has PubMed identifier	32668983
schema:publication	Exp Biol Med (Maywood)
resource representing a document's body	covid:5ce97aacf236a9cbac4393e43c83488effb9b191#body_text
is schema:about of	named entity 'reverse transcribed' named entity 'continuous testing' named entity 'SARS-CoV-2' named entity 'protocol' named entity 'saliva' named entity 'Direct' named entity 'SARS-CoV-2' named entity 'Original' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'PROVIDES' named entity 'lockdown' named entity 'purification' named entity 'PCR' named entity 'transition' named entity 'protocol' named entity 'RNA' named entity 'PCR' named entity 'SARS-CoV-2' named entity 'continuous testing' named entity 'nose and throat' named entity 'SARS-CoV-2' named entity 'saliva samples' named entity 'SARS-CoV-2' named entity 'primers' named entity 'true negative rate' named entity 'Fisher Scientific' named entity 'COVID' named entity 'viruses' named entity 'COVID' named entity 'DNA amplification' named entity 'RNA' named entity 'COVID' named entity 'exit strategy' named entity 'infection' named entity 'COVID' named entity 'saliva samples' named entity 'fluorescent' named entity 'heat source' named entity 'lab equipment' named entity 'thermometer' named entity 'RT-qPCR' named entity 'influenza' named entity 'Bio-Rad' named entity 'Proteinase' named entity 'BGI' named entity 'Rambam Health Care Campus' named entity 'RNA' named entity 'protons' named entity 'SARS-CoV-2' named entity 'RHCC' named entity 'RT-qPCR' named entity 'RNA' named entity 'nucleic acids' named entity 'nucleic acid detection' named entity 'SARS-CoV-2' named entity 'clinical diagnostic' named entity 'Colorimetric' named entity 'positive control' named entity 'detection limit' named entity 'viral RNA' named entity 'RT-qPCR' named entity 'true positive rate' named entity 'COVID-19 pandemic' named entity 'RHCC' named entity 'chemical reagents' named entity 'phenol red' named entity 'colorimetric'

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