About: Expression of Helicobacter pylori cag12 gene in Lactococcus lactis MG1363 and its oral administration to induce systemic anti-Cag12 immune response in mice

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About: Expression of Helicobacter pylori cag12 gene in Lactococcus lactis MG1363 and its oral administration to induce systemic anti-Cag12 immune response in mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression of Helicobacter pylori cag12 gene in Lactococcus lactis MG1363 and its oral administration to induce systemic anti-Cag12 immune response in mice
Creator	Chae, Ha Do, Youn Kim, Kim, Su-Jung Young, Ho
Source	Medline; PMC
abstract	To develop an oral vaccine against Helicobacter pylori infection, we have expressed the H. pyloricag12 (HP0532) gene, encoding the outer membrane protein Cag12 (31 kDa), in a live delivery vehicle Lactococcus lactis. The cag12 gene was amplified by polymerase chain reaction (PCR) using the genomic DNA of H. pylori K51 isolated from Korean patients. DNA sequence analysis revealed that the cag12 gene of H. pylori K51 has 98.1 and 97.4% identity with individual cag12 genes of the H. pylori 26695 and J99, respectively. The GST–Cag12 fusion protein, produced using the Escherichia coli expression system, was used to raise a rat polyclonal anti-Cag12 antibody. The PCR-amplified cag12 gene of H. pylori K51 was cloned in the E. coli–L. lactis shuttle vector (pMG36e) and transformed into L. lactis. Western blot analysis demonstrated that the Cag12 protein was expressed in the L. lactis transformant, with a maximum level at the log phase without extracelluar secretion. The oral administration of the transformant into mice resulted in the generation of the anti-Cag12 antibody in serum in two out of five cases. These results suggest that the recombinant L. lactis, which expresses Cag12, may be applicable as an oral vaccine to induce protective immunity against H. pylori.
has issue date	2006-04-04(xsd:dateTime)
bibo:doi	10.1007/s00253-005-0285-2
bibo:pmid	16586107
has license	no-cc
sha1sum (hex)	5c723f0797c46cc726f434637cbd6fca28db643d
schema:url	https://doi.org/10.1007/s00253-005-0285-2
resource representing a document's title	Expression of Helicobacter pylori cag12 gene in Lactococcus lactis MG1363 and its oral administration to induce systemic anti-Cag12 immune response in mice
has PubMed Central identifier	PMC7079898
has PubMed identifier	16586107
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:5c723f0797c46cc726f434637cbd6fca28db643d#body_text
is schema:about of	named entity 'LACTOCOCCUS LACTIS' named entity 'L. lactis' named entity 'isolated' named entity 'INDUCE' named entity 'MICE' named entity 'KDA' named entity 'ORAL ADMINISTRATION' named entity 'HELICOBACTER PYLORI INFECTION' named entity 'RAT' named entity 'ANTIBODY' named entity 'PHASE' named entity 'TRANSFORMANT' named entity 'POLYMERASE CHAIN REACTION' named entity 'ENCODING' named entity 'LIVE' named entity '26695' named entity 'CLONED' named entity 'GENERATION' named entity 'THESE' named entity 'LOG' named entity 'K51' named entity 'GENE' named entity 'ITS' named entity 'PROCESS ENGINEERING' named entity 'PCR' named entity 'ESCHERICHIA COLI' named entity 'HELICOBACTER PYLORI' named entity 'EXPRESSION SYSTEM' named entity 'SUGGEST' named entity 'REVEALED' named entity 'VEHICLE' named entity 'PROTEIN ' named entity 'POLYCLONAL' named entity 'USING' named entity 'EXPRESSION' named entity 'PRODUCTS' named entity 'LEVEL' named entity 'DEMONSTRATED' named entity 'E. COLI' named entity 'EXPRESSED' named entity 'ORAL VACCINE' named entity 'KOREAN' named entity 'J99' named entity 'RECOMBINANT' named entity 'DELIVERY' named entity 'SHUTTLE VECTOR' named entity 'FUSION PROTEIN' named entity 'PATIENTS' named entity 'MICE' named entity 'INDUCE' named entity 'SYSTEMIC' named entity 'ORAL ADMINISTRATION' named entity 'AMPLIFIED' named entity 'HAVE' named entity 'RESULTS' named entity 'SERUM' named entity 'INDIVIDUAL' named entity 'IDENTITY' named entity 'SECRETION' named entity 'CASES' named entity 'OUTER MEMBRANE PROTEIN' named entity 'USED' named entity 'MAXIMUM' named entity 'DNA SEQUENCE ANALYSIS' named entity 'GENE' named entity 'PROTECTIVE IMMUNITY' named entity 'H. PYLORI' named entity 'APPLICABLE' named entity 'IMMUNE RESPONSE' named entity 'ISOLATED' named entity 'GENES' named entity 'WESTERN BLOT ANALYSIS' named entity 'TRANSFORMED' named entity 'LACTOCOCCUS LACTIS' named entity 'GENOMIC DNA' named entity 'MAY BE' covid:arg/5c723f0797c46cc726f434637cbd6fca28db643d

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