About: The coronavirus proofreading exoribonuclease mediates extensive viral recombination

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The coronavirus proofreading exoribonuclease mediates extensive viral recombination
Creator	Routh, Andrew Lu, Xiaotao Agostini, Maria Chappell, James Pruijssers, Andrea Stevens, Laura Denison, Anderson-Daniels, Jordan Gribble, Jennifer
source	BioRxiv
abstract	Coronaviruses (CoVs) emerge as zoonoses and cause severe disease in humans, demonstrated by the SARS-CoV-2 (COVID-19) pandemic. RNA recombination is required during normal CoV replication for subgenomic mRNA (sgmRNA) synthesis and generates defective viral genomes (DVGs) of unknown function. However, the determinants and patterns of CoV recombination are unknown. Here, we show that divergent β-CoVs SARS-CoV-2, MERS-CoV, and murine hepatitis virus (MHV) perform extensive RNA recombination in culture, generating similar patterns of recombination junctions and diverse populations of DVGs and sgmRNAs. We demonstrate that the CoV proofreading nonstructural protein (nsp14) 3’-to-5’ exoribonuclease (nsp14-ExoN) is required for normal CoV recombination and that its genetic inactivation causes significantly decreased frequency and altered patterns of recombination in both infected cells and released virions. Thus, nsp14-ExoN is a key determinant of both high fidelity CoV replication and recombination, and thereby represents a highly-conserved and vulnerable target for virus inhibition and attenuation.
has issue date	2020-04-24(xsd:dateTime)
bibo:doi	10.1101/2020.04.23.057786
has license	biorxiv
sha1sum (hex)	5b672aba947e25f56d1ee5a8a446a32ee64e8684
schema:url	https://doi.org/10.1101/2020.04.23.057786
resource representing a document's title	The coronavirus proofreading exoribonuclease mediates extensive viral recombination
schema:publication	bioRxiv
resource representing a document's body	covid:5b672aba947e25f56d1ee5a8a446a32ee64e8684#body_text
is schema:about of	named entity 'RNA' named entity 'subgenomic mRNA' named entity 'Cell lines' named entity 'subgenomic RNA' named entity 'virus' named entity 'HCoV-229E' named entity 'clone' named entity 'CoV' named entity 'SARS-CoV-2' named entity 'Nanopore' named entity 'MERS-CoV' named entity 'RNA' named entity 'recombination' named entity 'RNA' named entity 'genome' named entity 'SARS-CoV-2' named entity 'proofreading' named entity '3' untranslated' named entity '5' UTR' named entity '0.45' named entity 'RNA sequences' named entity 'recombination' named entity 'humans' named entity 'Coronaviruses' named entity 'COVID-19' named entity 'zoonoses' named entity 'coronavirus' named entity 'murine' named entity 'RNA' named entity 'diverse populations' named entity 'Oxford Nanopore Technologies' named entity 'MHV' named entity 'MERS-CoV' named entity 'genome' named entity 'CoV' named entity 'recombination' named entity 'astrocytoma' named entity 'recombination' named entity 'highly-conserved' named entity 'murine' named entity 'RNA-seq' named entity 'hepatitis virus' named entity 'recombination' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'proofreading' named entity 'recombination' named entity 'cytopathic effect' named entity 'CoV' named entity 'genome' named entity 'RNA' named entity 'genome' named entity 'ExoN' named entity 'genome' named entity 'nucleotides' named entity 'RNA' named entity 'RNA molecules' named entity 'Nanopore' named entity 'RNA' named entity 'recombination' named entity 'high fidelity' named entity 'passaged' named entity '3'-UTR' named entity 'virions' named entity 'RNA' named entity 'genomes' named entity 'RNA' named entity 'recombination' named entity 'MHV' named entity 'RNA' named entity 'Nanopore' named entity 'exoribonuclease' named entity 'nucleoside analogs' named entity 'gene' named entity 'virulence' named entity 'isoforms'

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