About: In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination

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About: In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination
Creator	Cho, Kyoung-Oh Jeong, Jae-Ho Park, Su-Jin Kwon, Hyung-Jun Rho, Mun-Chual Jeong, Jae Lee, Seung Lee, Woo Lim, Jae Ryu, Young Jung, Kyungsook
topic	covid:5b5b47da22809b8c85f26dbfe9f4e7444354ed05#this
source	Elsevier; Medline; PMC
abstract	In this study, we evaluated the anti-reovirus activity of kuraridin isolated from the roots of Sophora flavescens. In particular, we focused on whether this property is attributable to direct inhibition of reovirus attachment and/or inhibition of viral replication with the aid of time-of-addition (pre-treatment, simultaneous treatment, and post-treatment) experiments. No significant antiviral activity of kuraridin was detected in the pre-treatment assay. In the simultaneous assay, the 50% effective inhibitory concentrations (EC(50)) of kuraridin were 15.3–176.9 μM against human type 1–3 reoviruses (HRV1–3) and Korean porcine reovirus (PRV). Kuraridin completely blocked binding of viral sigma 1 protein to sialic acids at concentrations lower than 82.5 μM in the hemagglutination inhibition assay. Moreover, kuraridin inhibited HRV1–3 and PRV viral replication with EC(50) values of 14.0–62.0 μM. Quantitative real-time PCR analysis disclosed strong suppression of reovirus RNA synthesis at the late stage (18 h) of virus replication by kuraridin. The viral yields of kuraridin-treated cells were significantly reduced at 24 h post-infection, compared with DMSO-treated cells. Our results collectively suggest that kuraridin inhibits virus adsorption and replication by inhibiting hemagglutination, viral RNA and protein synthesis and virus shedding, supporting its utility as a viable candidate antiviral drug against reoviruses.
has issue date	2015-04-30(xsd:dateTime)
bibo:doi	10.1016/j.jphs.2015.04.007
bibo:pmid	26076959
has license	no-cc
sha1sum (hex)	5b5b47da22809b8c85f26dbfe9f4e7444354ed05
schema:url	https://doi.org/10.1016/j.jphs.2015.04.007
resource representing a document's title	In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination
has PubMed Central identifier	PMC7172900
has PubMed identifier	26076959
schema:publication	J Pharmacol Sci
resource representing a document's body	covid:5b5b47da22809b8c85f26dbfe9f4e7444354ed05#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/5b5b47da22809b8c85f26dbfe9f4e7444354ed05
is schema:about of	named entity 'replication' named entity 'activity' named entity 'inhibition' named entity 'collectively' named entity 'In vitro' named entity 'license' named entity 'ADDITION' named entity 'UTILITY' named entity 'SIGNIFICANT' named entity 'PROTEIN SYNTHESIS' named entity 'POST-TREATMENT' named entity 'PROPERTY' named entity 'REPLICATION' named entity 'PRE-TREATMENT' named entity 'VIRUS SHEDDING' named entity 'LOWER' named entity 'COMPLETELY' named entity 'VIRAL' named entity 'ASSAY' named entity 'INHIBITED' named entity 'VIRUS REPLICATION' named entity 'AID' named entity 'TYPE' named entity 'COMPARED' named entity 'ANTIVIRAL DRUG' named entity 'ITS' named entity 'EVALUATED' named entity 'sigma' named entity 'protein synthesis' named entity 'viral' named entity 'concentrations' named entity 'direct' named entity 'virus' named entity 'assay' named entity 'inhibited' named entity 'inhibition' named entity 'http' named entity 'PRV' named entity 'reovirus' named entity 'viral replication' named entity 'PRV' named entity 'reovirus' named entity 'assay' named entity 'reovirus' named entity 'reovirus' named entity 'hemagglutination' named entity 'virus replication' named entity 'real-time PCR' named entity 'by-nc-nd' named entity 'inhibits' named entity 'reovirus' named entity 'MeOH' named entity '0.01' named entity 'angiogenesis' named entity 'MOI' named entity 'infection' named entity 'light microscopy' named entity 'diarrhea' named entity 'EtOAc' named entity 'livestock' named entity 'propyl gallate' named entity 'Type 2' named entity 'host cell' named entity 'viral replication cycle' named entity 'adsorption' named entity 'EtOAc' named entity 'PRV' named entity 'RNA' named entity 'Applied Biosystems'

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