About: Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples

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About: Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples
Creator	Berger, Annemarie Aldabbagh, Souhaib Ciesek, Sandra Panning, Marcus Hengel, Hartmut Huzly, Daniela Eis-Hübinger, Anna Liebert, Uwe Widera, Marek Hönemann, Mario Jj, Wenzel Marx, Benjamin Schmidt, Barbara Streeck, Hendrik Ug, Liebert Wenzel, Jürgen
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: A novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in China in late 2019 and subsequently caused a pandemic. Surveillance is important to better appreciate this evolving pandemic and to longitudinally monitor the effectiveness of public health measures. OBJECTIVES: We aimed to provide a rapid, easy to establish and costeffective laboratory-based surveillance tool for SARS-CoV-2. Study design: We used minipools of RNA prepared from nucleic acid extractions of routine respiratory samples. We technically validated the assay and distributed the protocol within an informal network of five German university laboratories. RESULTS: We tested a total of 70 minipools resembling 700 samples shortly before the upsurge of cases in Germany from 17.02.2020- 10.03.2020. One minipool reacted positive and after resolution one individual sample tested SARS-COV-2 positive. This sample was from a hospitalized patient not suspected of having contracted SARS-CoV-2. CONCLUSIONS: Our approach of a laboratory-based surveillance for SARSCoV-2 using minipools proved its concept is easily adaptable and resource-saving. It might assist not only public health laboratories in SARS-CoV-2 surveillance.
has issue date	2020-04-22(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2020.104381
bibo:pmid	32344319
has license	no-cc
sha1sum (hex)	5b39934ddb3201bd094fc491cd905499be787119
schema:url	https://doi.org/10.1016/j.jcv.2020.104381
resource representing a document's title	Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples
has PubMed Central identifier	PMC7175872
has PubMed identifier	32344319
schema:publication	J Clin Virol
resource representing a document's body	covid:5b39934ddb3201bd094fc491cd905499be787119#body_text
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