About: Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor

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About: Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor
Creator	Wang, Jing Zhou, Lei Yang, Ruifu Wang, Hong Hu, Kongxin Guo, Zhaobiao Liu, Haihong Song, Yajun Huang, Huijie Huang, Lihua Yan, Zhongqiang Zhao, Yongkai
source	Elsevier; Medline; PMC
abstract	Abstract Up-converting phosphor technology (UPT)-based lateral-flow immunoassay has been developed for quantitative detection of Yersinia pestis rapidly and specifically. In this assay, 400nm up-converting phosphor particles were used as the reporter. A sandwich immumoassay was employed by using a polyclonal antibody against F1 antigen of Y. pestis immobilized on the nitrocellulose membrane and the same antibody conjugated to the UPT particles. The signal detection of the strips was performed by the UPT-based biosensor that could provide a 980nm IR laser to excite the phosphor particles, then collect the visible luminescence emitted by the UPT particles and finally convert it to the voltage as a signal. V T and V C stand for the multiplied voltage units for the test and the control line, respectively, and the ratio V T/V C is directly proportional to the number of Y. pestis in a sample. We observed a good linearity between the ratio and logCFU/ml of Y. pestis above the detection limit, which was approximately 104 CFU/ml. The precision of the intra- and inter-assay was below 15% (coefficient of variation, CV). Cross-reactivity with related Gram-negative enteric bacteria was not found. The UPT-LF immunoassay system presented here takes less than 30min to perform from the sample treatment to the data analysis. The current paper includes only preliminary data concerning the biomedical aspects of the assay, but is more concentrated on the technical details of establishing a rapid manual assay using a state-of-the-art label chemistry.
has issue date	2006-12-07(xsd:dateTime)
bibo:doi	10.1016/j.snb.2006.01.029
bibo:pmid	32288237
has license	els-covid
sha1sum (hex)	5b01c0f218d069ed312bf2ab678306f047da7734
schema:url	https://doi.org/10.1016/j.snb.2006.01.029
resource representing a document's title	Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor
has PubMed Central identifier	PMC7125792
has PubMed identifier	32288237
schema:publication	Sensors and Actuators B: Chemical
resource representing a document's body	covid:5b01c0f218d069ed312bf2ab678306f047da7734#body_text
is schema:about of	named entity 'BASED' named entity 'DETECTION' named entity 'INTRA' named entity 'NOT FOUND' named entity 'USING' named entity 'STRIPS' named entity 'CHEMISTRY' named entity 'NITROCELLULOSE MEMBRANE' named entity 'ANTIGEN' named entity 'IMMUNOASSAY' named entity 'DETECTION' named entity 'GOOD' named entity '400' named entity 'DATA ANALYSIS' named entity 'COLLECT' named entity 'PRECISION' named entity 'CONVERT' named entity 'PROVIDE' named entity 'CURRENT' named entity 'TREATMENT' named entity 'ENTERIC BACTERIA' named entity 'NEGATIVE' named entity 'GRAM-' named entity 'UPT' named entity 'RAPID' named entity 'LASER' named entity 'STATE' named entity 'BACTERIA ' named entity 'PRELIMINARY DATA' named entity 'DETECTION LIMIT' named entity 'SAMPLE' named entity 'LESS THAN' named entity 'ASSAY' named entity 'BIOMEDICAL' named entity 'YERSINIA PESTIS' named entity 'PHOSPHOR' named entity 'LATERAL' named entity 'BIOSENSOR' named entity 'IMMUNOASSAY' named entity 'CONVERTING' named entity '30 MIN' named entity 'PERFORM' named entity 'TECHNICAL' named entity 'DETAILS' named entity 'V T' named entity 'OF-' named entity 'CFU' named entity 'PHOSPHOR' named entity 'REACTIVITY' named entity 'MANUAL' named entity 'SYSTEM' named entity 'RATIO' named entity 'LATERAL' named entity 'PERFORMED BY' named entity 'YERSINIA PESTIS' named entity 'LOG' named entity 'RELATED' named entity 'ESTABLISHING' named entity 'LABEL' named entity 'LINE' named entity 'QUANTITATIVE' named entity 'TECHNOLOGY' named entity 'FLOW' named entity 'CONVERTING' named entity 'VOLTAGE' named entity 'APPROXIMATELY' named entity 'BIOSENSOR' named entity 'STAND' named entity 'FLOW' named entity 'VARIATION'

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