About: Human cytomegalovirus and Herpes Simplex type I virus can engage RNA polymerase I for transcription of immediate early genes

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Human cytomegalovirus and Herpes Simplex type I virus can engage RNA polymerase I for transcription of immediate early genes
Creator	Ananthaseshan, Sharan Bartek, Jiri Raiss, Sina Söderberg-Naucler, Cecilia Wilhelmi, Vanessa Kostopoulou, Ourania Lindström, Mikael
Source	Medline; PMC
abstract	Human cytomegalovirus (HCMV) utilizes RNA polymerase II to transcribe viral genes and produce viral mRNAs. It can specifically target the nucleolus to facilitate viral transcription and translation. As RNA polymerase I (Pol I)-mediated transcription is active in the nucleolus, we investigated the role of Pol I, along with relative contributions of the human Pol II and Pol III, to early phases of viral transcription in HCMV infected cells, compared with Herpes Simplex Virus-1 (HSV-1) and Murine cytomegalovirus (MCMV). Inhibition of Pol I with siRNA or the Pol I inhibitors CX-5461 or Actinomycin D (5nM) resulted in significantly decreased IE and pp65 mRNA and protein levels in human fibroblasts at early times post infection. This initially delayed replication was compensated for later during the replication process, at which stage it didn’t significantly affect virus production. Pol I inhibition also reduced HSV-1 ICP0 and gB transcripts, suggesting that some herpesviruses engage Pol I for their early transcription. In contrast, inhibition of Pol I failed to affect MCMV transcription. Collectively, our results contribute to better understanding of the functional interplay between RNA Pol I-mediated nucleolar events and the Herpes viruses, particularly HCMV whose pathogenic impact ranges from congenital malformations and potentially deadly infections among immunosuppressed patients, up to HCMV’s emerging oncomodulatory role in human tumors.
has issue date	2017-10-29(xsd:dateTime)
bibo:doi	10.18632/oncotarget.22106
bibo:pmid	29228551
has license	cc-by
sha1sum (hex)	5a5a25d733b227ba661f3ba8f69af5886999f02c
schema:url	https://doi.org/10.18632/oncotarget.22106
resource representing a document's title	Human cytomegalovirus and Herpes Simplex type I virus can engage RNA polymerase I for transcription of immediate early genes
has PubMed Central identifier	PMC5722503
has PubMed identifier	29228551
schema:publication	Oncotarget
resource representing a document's body	covid:5a5a25d733b227ba661f3ba8f69af5886999f02c#body_text
is schema:about of	named entity 'compensated' named entity 'transcription' named entity 'herpesviruses' named entity 'replication' named entity 'levels' named entity 'RNA POLYMERASE I' named entity 'VIRUSES' named entity 'PROTEIN LEVELS' named entity 'PP65' named entity 'THEIR' named entity 'HCMV' named entity 'AFFECT' named entity 'LATER' named entity 'ROLE' named entity 'ENGAGE' named entity 'HUMAN' named entity 'UNDERSTANDING' named entity 'RESULTS' named entity 'ENGAGE' named entity 'INITIALLY' named entity 'REPLICATION' named entity 'TRANSCRIPTS' named entity 'MCMV' named entity 'HERPESVIRUSES' named entity 'INFECTIONS' named entity 'TIMES' named entity 'INHIBITION' named entity 'MEDIATED' named entity 'BETTER' named entity 'TRANSCRIPTION' named entity 'POL I' named entity '27S' named entity 'NUCLEOLAR' named entity 'EARLY' named entity 'MRNA' named entity 'REPLICATION PROCESS' named entity 'INFECTION' named entity 'IMMUNOSUPPRESSED PATIENTS' named entity 'DELAYED' named entity 'EVENTS' named entity 'PATHOGENIC' named entity 'OUR' named entity 'FUNCTIONAL' named entity 'ACTINOMYCIN D' covid:arg/5a5a25d733b227ba661f3ba8f69af5886999f02c named entity 'IMMEDIATE EARLY GENES' named entity 'IMPACT' named entity 'CONTRIBUTE ' named entity 'TRANSCRIPTION' named entity 'DECREASED' named entity 'HERPES SIMPLEX TYPE I' named entity 'HUMAN CYTOMEGALOVIRUS' named entity 'COMPENSATED' named entity 'ICP0' named entity 'TUMORS' named entity 'HSV-1' named entity 'HERPES' named entity 'FAILED' named entity 'POST' named entity 'UP TO' named entity 'CONTRAST' named entity 'VIRUS' named entity 'REDUCED' named entity 'FIBROBLASTS' named entity 'STAGE' named entity 'VIRUS PRODUCTION' named entity 'CONGENITAL MALFORMATIONS' named entity 'production' named entity 'Nucleus' named entity 'formaldehyde' named entity 'patients' named entity 'pathogenic' named entity 'virus' named entity 'human tumors' named entity 'functional'

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