About: Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2)

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2)
Creator	Xiao, Fengxia Burns, Kevin
Source	Medline; PMC
abstract	Angiotensin-converting enzyme 2 (ACE2) is a recently described member of the renin-angiotensin system that hydrolyzes angiotensin (Ang) II to Ang-(1-7), and may thereby protect against cardiovascular and renal diseases. ACE2 is a type 1 integral membrane protein and contains a catalytically active ectodomain that can be shed from the cell surface into the extracellular space, via cleavage by a disintegrin and metalloproteinase-17 (ADAM-17). ACE2 enzymatic activity and protein can be detected in biological fluids, including urine, plasma, and conditioned cell culture media. We present a detailed method for measurement of ACE2 activity in biological fluids, using hydrolysis of an intramolecularly quenched fluorogenic ACE2 substrate, in the absence or presence of the ACE2 inhibitors MLN-4760 or DX600. Recombinant human or mouse ACE2 is used to generate standard curves for this assay, with ACE2 detection ranging from 1.56 to 50 ng/ml. While MLN-4760 potently inhibits the activity of both human and mouse ACE2, DX600 (linear form) only effectively blocks human ACE2 activity in this assay. In biological samples of human and mouse urine, cell culture medium from mouse proximal tubular cells, and mouse plasma, the mean intra- and inter-assay coefficients of variation (CVs) of the assay range from 1.43 to 4.39 %, and from 7.01 to 13.17 %, respectively. We present data on the time and substrate concentration dependence of the assay, and show that exogenous d -glucose, creatinine, urea, and albumin do not interfere with its performance. In biological fluids, this assay is a simple and reliable method to study the role of ACE2 and its shed fragments in cardiovascular and renal diseases.
has issue date	2017-01-24(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-6625-7_8
bibo:pmid	28116710
has license	no-cc
sha1sum (hex)	594b85eae1a25e4ac2f1d23a17d8b6a93c01c92d
schema:url	https://doi.org/10.1007/978-1-4939-6625-7_8
resource representing a document's title	Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2)
has PubMed Central identifier	PMC7121061
has PubMed identifier	28116710
schema:publication	Hypertension
resource representing a document's body	covid:594b85eae1a25e4ac2f1d23a17d8b6a93c01c92d#body_text
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