About: High level expression of soluble glycoproteins in the allantoic fluid of embryonated chicken eggs using a Sendai virus minigenome system

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About: High level expression of soluble glycoproteins in the allantoic fluid of embryonated chicken eggs using a Sendai virus minigenome system Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High level expression of soluble glycoproteins in the allantoic fluid of embryonated chicken eggs using a Sendai virus minigenome system
Creator	Cano, Olga Corral, Teresa García-Barreno, Blanca Mottet, Geneviève Roux, Laurent Calder, Lesley Skehel, John Melero, José Ver, Lorena
topic	covid:58f68e82f134cf3549778fca86251ee61975b596#this
Source	Medline; PMC
abstract	BACKGROUND: Embryonated chicken eggs have been used since the mid-20th century to grow a wide range of animal viruses to high titers. However, eggs have found so far only limited use in the production of recombinant proteins. We now describe a system, based on a Sendai virus minigenome, to produce large amounts of heterologous viral glycoproteins in the allantoic cavity of embryonated eggs. RESULTS: Soluble forms of human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV) fusion (F) proteins, devoid of their transmembrane and cytoplasmic domains, were produced in allantoic fluids using the Sendai minigenome system. The first step was rescuing in cell cultures Sendai virus minigenomes encoding the proteins of interest, with the help of wild type Sendai virus. The second step was propagating such recombinant defective viruses, together with the helper virus, in the allantoic cavity of chicken embryonated eggs, and passage to optimize protein production. When compared with the production of the same proteins in the culture supernatant of cells infected with vaccinia recombinants, the yield in the allantoic fluid was 5–10 fold higher. Mutant forms of these soluble proteins were easily constructed by site-directed mutagenesis and expressed in eggs using the same approach. CONCLUSION: The simplicity and economy of the Sendai minigenome system, together with the high yield achieved in the allantoic fluid of eggs, makes it an attractive method to express soluble glycoproteins aimed for structural studies.
has issue date	2007-04-05(xsd:dateTime)
bibo:doi	10.1186/1472-6750-7-17
bibo:pmid	17411439
has license	cc-by
sha1sum (hex)	58f68e82f134cf3549778fca86251ee61975b596
schema:url	https://doi.org/10.1186/1472-6750-7-17
resource representing a document's title	High level expression of soluble glycoproteins in the allantoic fluid of embryonated chicken eggs using a Sendai virus minigenome system
has PubMed Central identifier	PMC1852797
has PubMed identifier	17411439
schema:publication	BMC Biotechnol
resource representing a document's body	covid:58f68e82f134cf3549778fca86251ee61975b596#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/58f68e82f134cf3549778fca86251ee61975b596
is schema:about of	named entity 'ALLANTOIC FLUID' named entity 'titers' named entity 'High' named entity 'eggs' named entity 'CHICKEN' named entity 'BACKGROUND' named entity 'EGGS' named entity 'FAR' named entity 'LARGE' named entity 'CAVITY' named entity 'FOUND' named entity 'HIGH' named entity 'PRODUCTION' named entity 'CHICKEN' named entity 'RANGE' named entity 'DESCRIBE' named entity 'ANIMAL VIRUSES' named entity 'MID' named entity 'AMOUNTS' named entity 'LIMITED USE' named entity 'GLYCOPROTEINS' named entity 'HAVE' named entity 'VIRAL' named entity 'BASED' named entity 'SENDAI VIRUS' named entity 'EGGS' named entity 'SYSTEM' named entity 'SOLUBLE' named entity 'HIGH LEVEL' named entity 'USING' named entity 'GLYCOPROTEINS' named entity 'EXPRESSION' named entity 'HETEROLOGOUS' named entity 'WIDE' named entity '20TH CENTURY' named entity 'SYSTEM' named entity 'RECOMBINANT PROTEINS' named entity 'USED' named entity 'GROW' named entity 'SENDAI VIRUS' named entity 'TO PRODUCE' covid:arg/58f68e82f134cf3549778fca86251ee61975b596 named entity 'wide range' named entity 'Embryonated' named entity 'glycoproteins' named entity 'production of recombinant proteins' named entity 'mid' named entity 'cleavage' named entity 'production of proteins' named entity 'viruses' named entity 'tissue culture' named entity 'animal viruses' named entity 'SeV' named entity 'expression systems' named entity 'T7 polymerase' named entity 'polymerase' named entity 'heterologous' named entity 'monomer' named entity 'nucleoprotein' named entity 'cDNA' named entity 'cDNA' named entity 'gene' named entity 'Maximal levels' named entity 'Munich' named entity 'glycoproteins' named entity 'Electron microscopy' named entity 'infection' named entity 'monoclonal antibody' named entity 'supernatant' named entity 'vector' named entity 'HRSV'

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