About: Aerosolized avian influenza virus by laboratory manipulations

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Aerosolized avian influenza virus by laboratory manipulations
Creator	Xia, Zhiping Li, Dan Gao, Feng Li, Zhiping Li, Lin Li, Jinsong Ma, Limin Zhang, Yandong
Source	Medline; PMC
abstract	BACKGROUND: Avian H5N1 influenza viruses present a challenge in the laboratory environment, as they are difficult to collect from the air due to their small size and relatively low concentration. In an effort to generate effective methods of H5N1 air removal and ensure the safety of laboratory personnel, this study was designed to investigate the characteristics of aerosolized H5N1 produced by laboratory manipulations during research studies. RESULTS: Normal laboratory procedures used to process the influenza virus were carried out independently and the amount of virus polluting the on-site atmosphere was measured. In particular, zootomy, grinding, centrifugation, pipetting, magnetic stirring, egg inoculation, and experimental zoogenetic infection were performed. In addition, common accidents associated with each process were simulated, including breaking glass containers, syringe injection of influenza virus solution, and rupturing of centrifuge tubes. A micro-cluster sampling ambient air pollution collection device was used to collect air samples. The collected viruses were tested for activity by measuring their ability to induce hemagglutination with chicken red blood cells and to propagate in chicken embryos after direct inoculation, the latter being detected by reverse-transcription PCR and HA test. The results showed that the air samples from the normal centrifugal group and the negative-control group were negative, while all other groups were positive for H5N1. CONCLUSIONS: Our findings suggest that there are numerous sources of aerosols in laboratory operations involving H5N1. Thus, laboratory personnel should be aware of the exposure risk that accompanies routine procedures involved in H5N1 processing and take proactive measures to prevent accidental infection and decrease the risk of virus aerosol leakage beyond the laboratory.
has issue date	2012-08-06(xsd:dateTime)
bibo:doi	10.1186/1743-422x-9-146
bibo:pmid	22866888
has license	cc-by
sha1sum (hex)	5759e5ac8b1cc3c13ba04aded6e588a059ad92a0
schema:url	https://doi.org/10.1186/1743-422x-9-146
resource representing a document's title	Aerosolized avian influenza virus by laboratory manipulations
has PubMed Central identifier	PMC3439333
has PubMed identifier	22866888
schema:publication	Virol J
resource representing a document's body	covid:5759e5ac8b1cc3c13ba04aded6e588a059ad92a0#body_text
is schema:about of	named entity 'H5N1' named entity 'size' named entity 'H5N1' named entity 'LABORATORY' covid:arg/5759e5ac8b1cc3c13ba04aded6e588a059ad92a0 named entity 'H5N1 INFLUENZA' named entity 'EFFECTIVE' named entity 'METHODS' named entity 'GENERATE' named entity 'CHARACTERISTICS' named entity 'THEIR' named entity 'AVIAN' named entity 'AVIAN INFLUENZA VIRUS' named entity 'SMALL SIZE' named entity 'DIFFICULT' named entity 'MANIPULATIONS' named entity 'TO INVESTIGATE' named entity 'CHALLENGE' named entity 'PRESENT' named entity 'H5N1' named entity 'TO COLLECT' named entity 'REMOVAL' named entity 'EFFORT' named entity 'SAFETY' named entity 'RESEARCH STUDIES' named entity 'BACKGROUND' named entity 'MANIPULATIONS' named entity 'ENSURE' named entity 'LOW CONCENTRATION' named entity 'AIR' named entity 'LABORATORY ENVIRONMENT' named entity 'DUE TO' named entity 'LABORATORY' named entity 'LABORATORY PERSONNEL' named entity 'INFLUENZA VIRUSES' named entity 'STUDY' named entity 'aerosolized' named entity 'laboratory' named entity 'avian influenza' named entity 'effective methods' named entity 'Aerosolized' named entity 'Rt-PCR' named entity 'influenza virus' named entity 'infection' named entity 'H5N1' named entity 'disposable syringe' named entity 'chicken' named entity 'infection' named entity 'Biosafety Level 3' named entity 'laboratory research' named entity 'viral infections' named entity 'H1N1 flu virus' named entity 'aerosols' named entity 'aerosols' named entity 'calcium' named entity 'infectivity' named entity 'biosafety' named entity 'PBS' named entity 'influenza virus' named entity 'Brucella' named entity 'Gram-negative bacteria' named entity 'Academy of Military Medical Sciences' named entity 'experiments on animals' named entity 'milliliter' named entity 'PBS' named entity 'centrifuge' named entity 'aerosol' named entity 'microtiter plates' named entity 'experimental research' named entity 'pressure drop' named entity 'Adsorption' named entity 'H5N1' named entity 'sealing wax' named entity 'influenza virus'

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