About: Proteomic analysis of up‐regulated proteins in human promonocyte cells expressing severe acute respiratory syndrome coronavirus 3C‐like protease

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About: Proteomic analysis of up‐regulated proteins in human promonocyte cells expressing severe acute respiratory syndrome coronavirus 3C‐like protease Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Proteomic analysis of up‐regulated proteins in human promonocyte cells expressing severe acute respiratory syndrome coronavirus 3C‐like protease
Creator	Li, Shih-Wein Lin, Cheng-Wen Wan, Lei Lai, Chien-Chen Tsai, Fuu-Jen Huang, Shiuan-Yi Jou, Ming-Jia
source	Medline; PMC
abstract	The pathogenesis of severe acute respiratory syndrome coronavirus (SARS CoV) is an important issue for treatment and prevention of SARS. Previously, SARS CoV 3C‐like protease (3CLpro) has been demonstrated to induce apoptosis via the activation of caspase‐3 and caspase‐9 (Lin, C. W., Lin, K. H., Hsieh, T. H., Shiu, S. Y. et al., FEMS Immunol. Med. Microbiol. 2006, 46, 375–380). In this study, proteome analysis of the human promonocyte HL‐CZ cells expressing SARS CoV 3CLpro was performed using 2‐DE and nanoscale capillary LC/ESI quadrupole‐TOF MS. Functional classification of identified up‐regulated proteins indicated that protein metabolism and modification, particularly in the ubiquitin proteasome pathway, was the main biological process occurring in SARS CoV 3CLpro‐expressing cells. Thirty‐six percent of identified up‐regulated proteins were located in the mitochondria, including apoptosis‐inducing factor, ATP synthase beta chain and cytochrome c oxidase. Interestingly, heat shock cognate 71‐kDa protein (HSP70), which antagonizes apoptosis‐inducing factor was shown to down‐regulate and had a 5.29‐fold decrease. In addition, confocal image analysis has shown release of mitochondrial apoptogenic apoptosis‐inducing factor and cytochrome c into the cytosol. Our results revealed that SARS CoV 3CLpro could be considered to induce mitochondrial‐mediated apoptosis. The study provides system‐level insights into the interaction of SARS CoV 3CLpro with host cells, which will be helpful in elucidating the molecular basis of SARS CoV pathogenesis.
has issue date	2007-04-04(xsd:dateTime)
bibo:doi	10.1002/pmic.200600459
bibo:pmid	17407183
has license	no-cc
sha1sum (hex)	565db03956b81045b5440091f53ca2c5a3ba4321
schema:url	https://doi.org/10.1002/pmic.200600459
resource representing a document's title	Proteomic analysis of up‐regulated proteins in human promonocyte cells expressing severe acute respiratory syndrome coronavirus 3C‐like protease
has PubMed Central identifier	PMC7167764
has PubMed identifier	17407183
schema:publication	Proteomics
resource representing a document's body	covid:565db03956b81045b5440091f53ca2c5a3ba4321#body_text
is schema:about of	named entity 'protease' named entity 'SARS' named entity 'HUMAN' named entity 'PROTEOMIC ANALYSIS' named entity 'severe acute respiratory syndrome coronavirus' named entity 'human' named entity '3C-like protease' named entity 'severe acute respiratory syndrome coronavirus' named entity 'time of flight' named entity 'up-regulated' named entity '3C-like protease' named entity 'FITC' named entity 'polyacrylamide gels' named entity 'SARS' named entity 'image analysis' named entity 'subcellular location' named entity '3CLpro' named entity '3CLpro' named entity 'caspase-3' named entity 'negative-strand' named entity 'HSP70' named entity 'RPMI' named entity 'peptides' named entity 'Apoptosis-inducing factor' named entity '3CLpro' named entity 'apoptosis' named entity 'signaling pathway' named entity 'mitochondria' named entity 'SARS' named entity '3CLpro' named entity 'SARS CoV' named entity 'heat shock' named entity 'replicase' named entity 'reactive oxygen species' named entity 'cilia' named entity 'antibodies' named entity 'elution' named entity '3CLpro' named entity 'digestion' named entity 'GE Healthcare' named entity 'heat shock' named entity 'molecular basis' named entity 'peptides' named entity 'SARS' named entity 'mAb' named entity 'regulatory proteins' named entity 'purine biosynthesis' named entity 'pH 8' named entity 'RNA genome' named entity 'cell apoptosis' named entity 'pathogenesis' named entity 'apoptosis' named entity '3CLpro' named entity 'apoptosis-inducing factor' named entity 'replicase' named entity 'Western blotting' named entity 'epithelial' named entity 'up-regulated' named entity '1, 2' named entity 'heat shock' named entity '2-DE' named entity 'beta-actin' named entity 'caspase-9' named entity 'protein' named entity 'protein' named entity 'protein' named entity 'Leica' named entity '3CLpro' named entity 'protein' named entity 'replicase' named entity 'transfected' named entity 'up-regulated' named entity 'heterogeneous nuclear ribonucleoproteins' named entity 'eluted' named entity 'protein biosynthesis'

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