About: Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells

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About: Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
Creator	Kato, Tatsuya Kumar Deo, Vipin Park, Enoch Takami, Yoshihiro
Source	Elsevier; Medline; PMC
abstract	Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) first emerged in 2012, and over 2000 infections and 800 deaths have been confirmed in 27 countries. However, to date, no commercial vaccine is available. In this study, structural proteins of MERS-CoV were expressed in silkworm larvae and Bm5 cells for the development of vaccine candidates against MERS-CoV and diagnostic methods. The spike (S) protein of MERS-CoV lacking its transmembrane and cytoplasmic domains (SΔTM) was secreted into the hemolymph of silkworm larvae using a bombyxin signal peptide and purified using affinity chromatography. The purified SΔTM forms small nanoparticles as well as the full-length S protein and has the ability to bind human dipeptidyl peptidase 4 (DPP4), which is a receptor of MERS-CoV. These results indicate that bioactive SΔTM was expressed in silkworm larvae. To produce MERS-CoV-like particles (MERS-CoV-LPs), the coexpression of spike proteins was performed in Bm5 cells and envelope (E) and membrane (M) proteins secreted E and M proteins extracellularly, suggesting that MERS-CoV-LPs may be formed. However, this S protein was not displayed on virus-like particles (VLPs) even though E and M proteins were secreted into the culture supernatant. By surfactant treatment and mechanical extrusion using S protein- or three structural protein-expressing Bm5 cells, S protein-displaying nanovesicles with diameters of approximately 100-200 nm were prepared and confirmed by immuno-TEM. The mechanical extrusion method is favorable for obtaining uniform recombinant protein-displaying nanovesicles from cultured cells. The purified SΔTM from silkworm larvae and S protein-displaying nanovesicles from Bm5 cells may lead to the development of nanoparticle-based vaccines against MERS-CoV and the diagnostic detection of MERS-CoV.
has issue date	2019-12-20(xsd:dateTime)
bibo:doi	10.1016/j.jbiotec.2019.10.007
bibo:pmid	31614169
has license	els-covid
sha1sum (hex)	5557f847ae1466924fdbb0c9c0adaa0d416680da
schema:url	https://doi.org/10.1016/j.jbiotec.2019.10.007
resource representing a document's title	Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
has PubMed Central identifier	PMC7114102
has PubMed identifier	31614169
schema:publication	Journal of Biotechnology
resource representing a document's body	covid:5557f847ae1466924fdbb0c9c0adaa0d416680da#body_text
is schema:about of	named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'secreted' named entity 'bind' named entity 'transmembrane' named entity '2015' named entity 'MERS-CoV' named entity 'MIMETIC' named entity 'USING' covid:arg/5557f847ae1466924fdbb0c9c0adaa0d416680da named entity 'STUDY' named entity 'DATE' named entity 'AVAILABLE' named entity 'DISPLAYING' named entity 'PREPARATION' named entity 'NANOPARTICLE' named entity 'LARVAE' named entity 'LPS' named entity 'RESULTS' named entity 'SECRETED' named entity 'SPIKE' named entity 'DIAMETERS' named entity 'DETECTION' named entity 'SURFACTANT' named entity 'PROTEIN ' named entity 'BIND' named entity 'HAVE' named entity 'METHODS' named entity 'LIKE' named entity 'M PROTEINS' named entity 'SILKWORM' named entity 'INSECT' named entity 'DOMAIN' named entity 'BINDING' named entity 'MAY BE' named entity 'FAVORABLE' named entity 'USING' named entity 'EXTRUSION' named entity 'TRANSMEMBRANE' named entity 'SMALL' named entity 'TREATMENT' named entity 'RECEPTOR' named entity 'PROTEINS' named entity 'AFFINITY CHROMATOGRAPHY' named entity 'PARTICLES' named entity 'LEAD' named entity 'DEATHS' named entity 'COUNTRIES' named entity 'CONFIRMED' named entity 'CONFIRMED BY' named entity '200' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'APPROXIMATELY' named entity 'TO PRODUCE' named entity 'STRUCTURAL PROTEIN' named entity 'FORMS' named entity 'VIRUS-LIKE PARTICLE' named entity 'S PROTEIN' named entity 'TARGETS' named entity 'COEXPRESSION' named entity 'COMMERCIAL' named entity 'METHOD' named entity '2015' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'STRUCTURAL PROTEINS' named entity 'CELLS' named entity 'THESE' named entity 'VACCINE' named entity 'ADDITION' named entity 'DISPLAYING' named entity 'S PROTEIN' named entity 'PROTEIN ' named entity 'M PROTEIN' named entity 'CORONAVIRUS' named entity 'BOMBYXIN' named entity 'RECOMBINANT PROTEIN' named entity 'LACKING' named entity 'ITS' named entity 'CULTURED CELLS'

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