About: Polyadenylation of genomic RNA and initiation of antigenomic RNA in a positive-strand RNA virus are controlled by the same cis-element

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Polyadenylation of genomic RNA and initiation of antigenomic RNA in a positive-strand RNA virus are controlled by the same cis-element Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Polyadenylation of genomic RNA and initiation of antigenomic RNA in a positive-strand RNA virus are controlled by the same cis-element
Creator	Andino, Raul Kuijpers, Judith Polacek, Charlotta Agol, Vadim Glaudemans, Dirk Melchers, Willem Van Kuppeveld, Frank Van Ooij, Mark
source	Medline; PMC
abstract	Genomes and antigenomes of many positive-strand RNA viruses contain 3′-poly(A) and 5′-poly(U) tracts, respectively, serving as mutual templates. Mechanism(s) controlling the length of these homopolymeric stretches are not well understood. Here, we show that in coxsackievirus B3 (CVB3) and three other enteroviruses the poly(A) tract is ∼80–90 and the poly(U) tract is ∼20 nt-long. Mutagenesis analysis indicate that the length of the CVB3 3′-poly(A) is determined by the oriR, a cis-element in the 3′-noncoding region of viral RNA. In contrast, while mutations of the oriR inhibit initiation of (−) RNA synthesis, they do not affect the 5′-poly(U) length. Poly(A)-lacking genomes are able to acquire genetically unstable AU-rich poly(A)-terminated 3′-tails, which may be generated by a mechanism distinct from the cognate viral RNA polyadenylation. The aberrant tails ensure only inefficient replication. The possibility of RNA replication independent of oriR and poly(A) demonstrate that highly debilitated viruses are able to survive by utilizing ‘emergence’, perhaps atavistic, mechanisms.
has issue date	2006-05-31(xsd:dateTime)
bibo:doi	10.1093/nar/gkl349
bibo:pmid	16738134
has license	no-cc
sha1sum (hex)	5543303ea370910fdff1ef7e9f77ddfe136ff683
schema:url	https://doi.org/10.1093/nar/gkl349
resource representing a document's title	Polyadenylation of genomic RNA and initiation of antigenomic RNA in a positive-strand RNA virus are controlled by the same cis-element
has PubMed Central identifier	PMC1474053
has PubMed identifier	16738134
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:5543303ea370910fdff1ef7e9f77ddfe136ff683#body_text
is schema:about of	named entity 'NOT WELL' named entity 'THESE' named entity 'MECHANISM' named entity '28A' named entity '28S' named entity 'GENOMES' named entity 'SERVING' named entity 'TRACTS' named entity 'RNA' named entity 'MUTUAL' covid:arg/5543303ea370910fdff1ef7e9f77ddfe136ff683 named entity 'INITIATION' named entity 'CONTAIN' named entity 'STRAND' named entity 'POSITIVE-STRAND RNA VIRUS' named entity 'CIS' named entity 'GENOMIC' named entity 'POSITIVE' named entity 'LENGTH' named entity 'CONTROLLING' named entity 'POLY' named entity 'TEMPLATES' named entity 'RNA VIRUSES' named entity 'UNDERSTOOD' named entity 'STRETCHES' named entity 'CONTROLLED BY' named entity 'ELEMENT' named entity 'POLYADENYLATION' named entity 'L-glutamine' named entity 'cloned' named entity 'N70' named entity 'VPg' named entity 'M199' named entity 'post-translational' named entity 'molecule' named entity 'RNAs' named entity 'viral RNA' named entity 'hexameric' named entity 'RNA' named entity 'homopolymeric' named entity 'RNA viruses' named entity 'RNA' named entity 'cis' named entity 'RNA' named entity 'deletions' named entity 'RNA viruses' named entity 'picornavirus' named entity 'open access' named entity 'nested PCR' named entity 'RNA' named entity 'SalI' named entity 'cis' named entity 'AAUAAA' named entity 'oligonucleotide' named entity 'stop codon' named entity 'RNA replication' named entity 'RNA' named entity 'adenosine' named entity 'virion' named entity 'PBS' named entity 'clone' named entity 'viral RNA' named entity 'phosphate-buffered saline' named entity 'Promega' named entity 'vector' named entity 'VPg' named entity 'polyadenylation' named entity 'helical' named entity 'error-prone' named entity 'Invitrogen' named entity 'viral RNA' named entity 'viruses' named entity 'PCR-based' named entity 'viral reproduction'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software